【摘要】 以干燥粉碎的剑叶玉簪花为材料，采用单因素试验结合正交试验优化其总黄酮提取工艺，结果表明：剑叶玉簪总黄酮最佳提取工艺为料液比1∶40、60℃、20 min、90%乙醇，得率达（6.114±0.056）mg/g。进一步抗氧化实验显示，剑叶玉簪总黄酮对ABTS⁺·的清除效果显著优于VC（p<0.05）;在浓度较低时，对DPPH·和·OH的清除能力优于VC（p<0.05）;铁离子还原能力在质量浓度≥0.1 mg·mL^-1时弱于VC（p<0.05）,质量浓度≤0.05 mg·mL^-1时与VC无显著差异（p>0.05）。运用CCK8法检测剑叶玉簪花总黄酮对胃癌BGC823细胞的抑制作用，结果显示其对BGC823细胞具有明显抑制活性，IC₅₀为23.84μg·mL^-1。综上，剑叶玉簪总黄酮展现出良好的抗氧化及抗肿瘤活性，具备潜在的应用价值。更多还原

【Abstract】 Using dried and pulverized Hosta ensata flowers as the raw material, the extraction process for total flavonoids was optimized through single-factor experiments combined with orthogonal tests.Results demonstrated that the optimal extraction conditions were a solid-liquid ratio of 1∶40,60 ℃,20 min, and 90% ethanol, achieving a yield of（6.114±0.056）mg/g.Further antioxidant assays revealed that the total flavonoids from Hosta ensata exhibited significantly stronger scavenging activity against ABTS+· compared to VC（p<0.05）.At lower concentrations, their DPPH· and ·OH scavenging capacities also surpassed those of VC（p<0.05）.However, their ferric ion-reducing power was weaker than VC when the concentration exceeded 0.1 mg·mL^-1（p<0.05） but showed no significant difference at concentrations≤0.05 mg·mL^-1（p>0.05）.The CCK-8 assay demonstrated that the total flavonoids significantly inhibited the proliferation of gastric cancer BGC823 cells, with an IC50 value of 23.84 μg·mL^-1.In conclusion, the total flavonoids from Hosta ensata exhibit remarkable antioxidant and antitumor activities, highlighting their potential for further development in pharmacological or nutraceutical applications.更多还原