【摘要】 【目的】明确木醋液调控草莓生长的分子机制。【方法】以草莓品种越心为材料，设置叶面喷施稻壳木醋液处理组，以喷施清水为对照组，通过整合表型分析、土壤理化性质检测、全株转录组及根际宏基因组测序结果，系统揭示木醋液促进草莓生长的调控网络。【结果】与对照组相比，木醋液处理组的草莓叶长和叶宽分别增加15.38%和7.86%，毛细根数量显著增多，表现出明显的促生作用。全株转录组鉴定到7 809个差异表达基因，其中木醋液处理组的上调基因主要涉及苯丙烷代谢、类黄酮合成、激素信号传导、光合作用等途径，这些基因通过激活激素信号通路、增强碳同化能力、促进木质素合成等共同驱动地上部与根系快速生长。宏基因组分析表明：木醋液能够选择性富集根际有益微生物，提高了慢生根瘤菌属(Bradyrhizobium)和鞘氨醇单胞菌属(Sphingomonas)相对丰度，同时显著降低假单胞菌属(Pseudomonas)相对丰度，并显著增强了碳水化合物代谢和氨基酸代谢等关键功能通路的活性，构建了高效的根际碳转化系统，进而实现草莓更快生长。【结论】本研究揭示了木醋液调控植物内源激素合成、增强光合效能以及重塑根际有益微生物群落结构的协同作用机制，为草莓绿色栽培技术研发提供了理论参考和实践指导。更多还原

【Abstract】 [Purpose]To clarify the molecular mechanism of wood vinegar regulating strawberry growth. [Methods]Using the strawberry variety ‘Yuexin’ as the material, a foliar spray treatment group with rice husk wood vinegar was set, with a water spray as the control group. By integrating phenotypic analysis, soil physicochemical property detection, and sequencing results of whole-plant transcriptome and rhizosphere metagenomic, the regulatory network of wood vinegar promoting strawberry growth was systematically elucidated. [Results]Compared with the control group, the strawberry leaf length and width in the wood vinegar treatment group increased by 15.38% and7.86%, respectively, and the number of capillary roots significantly increased, showing a significant promoting effect on growth. Whole-plant transcriptome analysis identified 7 809 differentially expressed genes. Up-regulated genes in wood vinegar-treated plants were primarily involved in pathways such as phenylpropanoid metabolism, flavonoid synthesis, hormone signaling and photosynthesis. These genes collectively drove the rapid growth of both the above-ground and root systems by activating hormone signaling pathways, enhancing the capacity for carbon assimilation, and promoting lignin synthesis. Metagenomic analysis revealed that wood vinegar selectively enriched beneficial rhizosphere microorganisms, increasing the relative abundance of Bradyrhizobium and Sphingomonas, while significantly reducing the relative abundance of Pseudomonas. Wood vinegar also significantly enhanced the activity of key functional pathways such as carbohydrate metabolism and amino acid metabolism, constructing an efficient rhizosphere carbon conversion system and accelerating the growth of strawberry. [Conclusion]The study reveals the synergistic mechanism of wood vinegar in regulating endogenous hormone synthesis, enhancing photosynthetic efficiency, and reshaping beneficial microbial community structure in the rhizosphere, providing theoretical reference and practical guidance for the development of green strawberry cultivation technology.更多还原