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1株多粘类芽孢杆菌NPDY05-8对玉米茎基腐病的防效
Identification and Biocontrol Efficacy of Paenibacillus polymyxa NPDY05-8 against Maize Stalk Rot
【摘要】 菌株NPDY05-8是由小麦赤霉病抗性鉴定圃土壤分离出的1株多粘类芽孢杆菌。该菌株对供试的19种病原真菌均有拮抗效果,为广谱抑菌菌株,对引起玉米茎基腐病的禾谷镰刀菌(Fusarium. graminearum)和腐皮镰刀菌(Fusarium. solani)的抑菌率分别为69.63%和65.55%。该菌株可在玉米根部及根际土壤中定殖,25 d后趋于稳定,根部及根际土壤定殖量均保持为2.0×10~4~2.5×10~4cfu/g。高通量测序探究菌株NPDY05-8施用后土壤真菌结构显示,该菌灌施后有效减少了子囊菌门和镰刀菌属等有害真菌的丰度,对玉米茎基腐病的盆栽防治效果达到95.99%,明显高于15%乙蒜素可湿性粉剂和15%多菌灵可湿性粉剂防效。
【Abstract】 The strain NPDY05-8 is a Paenibacillus polymyxa isolated from the soil of a wheat nursery used for screening resistance to Fusarium head blight. This strain exhibited antagonistic effects against all 19 tested pathogenic fungi, indicating it was a broad-spectrum antibacterial strain. Its inhibition rates against Fusarium graminearum and Fusarium solani, which caused maize stem rot disease, were 69.63% and 65.55%, respectively. This strain colonized the roots and rhizosphere soil of maize, and tends to stabilize after 25 days. The colonization amount in the roots and rhizosphere soil remained between(2.0-2.5)×10~4 cfu/g. High-throughput sequencing was used to investigate the soil fungal structure following the application of strain NPDY05-8. The results showed that the application of this strain effectively reduced the abundance of harmful fungi, such as Ascomycota and Fusarium. The biocontrol efficacy against maize stalk rot in potted plants was 95.99%, which was significantly higher than that of 15% ethylicin wettable powder and 15% carbendazim wettable powder.
【Key words】 Maize; Maize stalk rot; Paenibacillus polymyxa; Antifungal spectrum;
- 【文献出处】 玉米科学 ,Journal of Maize Sciences , 编辑部邮箱 ,2026年06期
- 【分类号】S435.131.4
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