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1株多粘类芽孢杆菌NPDY05-8对玉米茎基腐病的防效

Identification and Biocontrol Efficacy of Paenibacillus polymyxa NPDY05-8 against Maize Stalk Rot

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【作者】 邓云; 刘端华; 陈泳和; 杨秀娟; 林芳; 许卿; 甘霖; 谢冬容; 田大刚;

【Author】 DENG Yun;LIU Duan-hua;CHEN Yong-he;YANG Xiu-juan;LIN Fang;XU Qing;GAN Lin;XIE Dong-rong;TIAN Da-gang;Nanping Institute of Agricultural Sciences;Institute of Plant Protection, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests/Fujian Engineering Research Center for Green Prevention and Control of Crop Pests/Institute of Biotechnology, Fujian Academy of Agricultural Sciences;Nanping Seed Station;

【通讯作者】 谢冬容;

【机构】 福建省南平市农业科学研究所; 福建省农业科学院植物保护研究所/福建省作物有害生物监测与治理重点实验室/福建省作物有害生物绿色防控工程研究中心/福建省农业科学院生物技术研究所; 南平市种子站;

【摘要】 菌株NPDY05-8是由小麦赤霉病抗性鉴定圃土壤分离出的1株多粘类芽孢杆菌。该菌株对供试的19种病原真菌均有拮抗效果,为广谱抑菌菌株,对引起玉米茎基腐病的禾谷镰刀菌(Fusarium. graminearum)和腐皮镰刀菌(Fusarium. solani)的抑菌率分别为69.63%和65.55%。该菌株可在玉米根部及根际土壤中定殖,25 d后趋于稳定,根部及根际土壤定殖量均保持为2.0×10~4~2.5×10~4cfu/g。高通量测序探究菌株NPDY05-8施用后土壤真菌结构显示,该菌灌施后有效减少了子囊菌门和镰刀菌属等有害真菌的丰度,对玉米茎基腐病的盆栽防治效果达到95.99%,明显高于15%乙蒜素可湿性粉剂和15%多菌灵可湿性粉剂防效。

【Abstract】 The strain NPDY05-8 is a Paenibacillus polymyxa isolated from the soil of a wheat nursery used for screening resistance to Fusarium head blight. This strain exhibited antagonistic effects against all 19 tested pathogenic fungi, indicating it was a broad-spectrum antibacterial strain. Its inhibition rates against Fusarium graminearum and Fusarium solani, which caused maize stem rot disease, were 69.63% and 65.55%, respectively. This strain colonized the roots and rhizosphere soil of maize, and tends to stabilize after 25 days. The colonization amount in the roots and rhizosphere soil remained between(2.0-2.5)×10~4 cfu/g. High-throughput sequencing was used to investigate the soil fungal structure following the application of strain NPDY05-8. The results showed that the application of this strain effectively reduced the abundance of harmful fungi, such as Ascomycota and Fusarium. The biocontrol efficacy against maize stalk rot in potted plants was 95.99%, which was significantly higher than that of 15% ethylicin wettable powder and 15% carbendazim wettable powder.

【基金】 福建省科技计划引导性项目--多粘类芽孢杆菌对玉米茎基腐病的防治及应用(2024N0046);福建省属公益类科研院所专项“鲜食玉米镰孢菌病害成灾因子及防控技术研究”(2025R1063);福建省省属公益类科研院所基本科研专项(2021R1027001)
  • 【文献出处】 玉米科学 ,Journal of Maize Sciences , 编辑部邮箱 ,2026年06期
  • 【分类号】S435.131.4
  • 【下载频次】36
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