【摘要】 开发了一种近红外（NIR）荧光探针DCA-Tcf用于检测生物体系和食品样本中的二氧化硫衍生物(HSO₃^-/SO₃^2-).该探针以6-（二乙基氨基）-1,2-二氢环戊烯[b]苯并吡喃为荧光团,通过引入吸电子基,使其发射波长延伸至809 nm,突破了小分子探针通常难以逾越的800 nm波长限制.该发射波长可有效增强组织穿透深度并降低背景荧光干扰,使该探针在生物成像应用中更具优势.探针DCA-Tcf识别HSO₃^-/SO₃^2-表现出高选择性、快速响应（40 s）以及低至0.509μmol/L的检测限,并在真实水样和糖样中成功检测HSO₃^-,在活细胞荧光成像中展示出应用潜力.本研究开发的DCA-Tcf在食品安全检测和生物成像领域可成为一种有力工具.更多还原

【Abstract】 A novel near-infrared（NIR） fluorescent probe DCA-Tcf is developed for the detection of sulfur dioxide derivatives(HSO₃^-/SO₃^2-) in biological systems and food samples in this paper.Utilizing 6-（diethylamino）-1,2-dihydrocyclopentene-[b]benzopyran as the fluorophore,and incorporating electron-withdrawing groups,the emission wavelength of the probe is red-shifted to 809 nm,which surpasses the typical 800 nm barrier for small-molecule probes.The emission wavelength（809nm） can effectively enhance the tissue penetration depth and reduce the background fluorescence interference,making this probe more advantageous in biological imaging applications.Probe DCA-Tcf exhibits high selectivity,rapid response within40 s,and a detection limit as low as 0.509 μmol/L toward HSO₃^-/SO₃^2-.DCA-Tcf has been applied successfully to the detection of HSO₃^-in real water and sugar samples,and demonstrates promising potential for application in fluorescence imaging of living cells.The DCA-Tcf developed in this study can become a powerful tool in the fields of food safety detection and bioimaging.更多还原