【摘要】 为探明危害海南辣椒的病毒种类，2021年11月至2024年2月，从海南文昌、万宁、琼海、定安、临高、海口、澄迈和三亚共8个市县，采集疑似病毒病感染的一年生辣椒叶片样品184份。采用高通量测序和RT-PCR检测发现辣椒样品中存在9种病毒：黄瓜花叶病毒(cucumber mosaic virus, CMV)、辣椒脉黄化病毒1(pepper vein yellows virus 1, PeVYV-1)、甜椒内源RNA病毒(bell pepper endornavirus, BPEV)、辣椒褪绿病毒(capsicum chlorosis virus, CaCV)、甜椒脉斑驳病毒(pepper veinal mottle virus, PVMV)、辣椒潜隐病毒2(pepper cryptic virus 2, PCV2)、辣椒轻斑驳病毒(pepper mild mottle virus, PMMoV)、辣椒环斑病毒(chilli ringspot virus, ChiRSV)和辣椒脉斑驳病毒(chilli veinal mottle virus, ChiVMV),检出率分别为70.11%、 67.93%、 41.30%、 32.07%、 30.98%、 20.11%、 20.11%、 1.63%和1.09%。PeVYV-1、 BPEV、 CaCV和PCV2是首次在海南辣椒上检测到的病毒。样品中病毒的复合侵染现象普遍，复合侵染率为91.57%,以2～4种病毒复合侵染为主。通过RT-PCR扩增获得CaCV海南辣椒分离物的全基因组序列，基于基因组序列的相似性比对和进化树分析发现，该分离物与来自海南的蜘蛛兰分离物CaCV-Hainan和藿香蓟分离物CaCV-AC的基因组相似性最高，而且始终聚集在同一进化分支上。更多还原

【Abstract】 To identify the viral species infecting pepper plants in Hainan Province, a total of 184 leaf samples from annual pepper plants exhibiting suspected viral symptoms were collected between November 2021 and February 2024 from eight cities and counties, including Wenchang, Wanning, Qionghai, Ding’an, Lin’gao, Haikou, Chengmai and Sanya. Nine viruses including cucumber mosaic virus(CMV), pepper vein yellows virus 1(PeVYV-1), bell pepper endornavirus(BPEV), capsicum chlorosis virus(CaCV), pepper veinal mottle virus(PVMV), pepper cryptic virus 2(PCV2), pepper mild mottle virus(PMMoV), chilli ringspot virus(ChiRSV), and chilli veinal mottle virus(ChiVMV) were detected in the pepper samples by using small RNA deep sequencing and RT-PCR, with detection rates of 70.11%, 67.93%, 41.30%, 32.07%, 30.98%, 20.11%, 20.11%, 1.63%, and 1.09%, respectively. Among these, PeVYV-1, BPEV, CaCV and PCV2 were identified in Hainan pepper plants for the first time. Co-infection by multiple viruses was prevalent, with a co-infection rate of 91.57%, primarily involving two to four viruses. The genome sequence of CaCV isolate from Hainan pepper was obtained by RT-PCR, and the sequence similarity comparison and phylogenetic tree analysis based on the complete genome revealed that CaCV in this study had the highest genome similarity with the Hymenocallis americana isolate CaCV-Hainan and Ageratum conyzoides isolate CaCV-AC from Hainan, and clustered on the same evolutionary branch.更多还原