【摘要】 为了确定山东某鸭场鸭群出现的呼吸道症状以及产蛋量下降的病原，试验对发病鸭的组织样品进行病原分离和PCR鉴定，研究该病原的遗传进化特征及致病特性。结果：禽偏肺病毒(aMPV)核酸阳性，未检测出其他鸭的病原。接种样品的SPF鸡胚盲传4代后生长迟缓、胚体出血，随后将胚体研磨液接种Vero细胞连续盲传7代后出现明显的细胞病变。对该分离株的G基因核苷酸序列进行同源性分析，显示与国内分离的C亚型参考株KC915036.1同源性最高，为96.8%,分离株与C亚型参考株属同一分支，表明分离株属于aMPV/C亚型。该病毒感染1日龄樱桃谷肉鸭出现轻微呼吸道症状，病毒可在鼻甲骨、喉头气管、肺脏中有效复制，感染后的肉鸭通过口咽排毒，排毒期为1～5 d。说明试验成功从疑似感染aMPV的种鸭病料中分离出一株aMPV,将其命名为APV-01。更多还原

【Abstract】 To determine the causative agent of concurrent respiratory diseases and egg production decline in a duck population at a Shandong farm,pathogenic isolation and PCR detection were conducted on tissue samples from clinically affected ducks.The results showed that the avian metapneumovirus(aMPV) nucleic acid was positive,with no otherduck pathogens detected.The SPF chicken embryos inoculated with the virus exhibited growth retardation and embryonic hemorrhage after four blind passages.Subsequent inoculation of the embryo homogenate into Vero cells induced significant cytopathic effects following seven serial blind passages.Homology analysis of the nucleotide sequence of the G gene revealed its highest identity(96.8%) with the Chinese reference strain KC915036.1 of subtype C.Phylogenetic analysis confirmed that the isolated strain clustered within the aMPV/C subtype,designated as APV-01.Experimental infection of 1-day-old Cherry Valley ducks with this isolate induced mild respiratory symptoms.Viral replication was detected in nasal turbinates,trachea,and lungs,with oropharyngeal virus shedding persisting for 1-5 days post-infection.These results confirmed,in the present study,a successful isolation of aMPV strain from clinical specimens of suspected aMPV-infected breederducks,designated as APV-01.更多还原