【摘要】 以黑皮冬瓜为原料，采用单因素及响应面试验优化冬瓜多糖提取工艺。考察了冬瓜多糖体外抗氧化活性、保湿性、美白活性。结果表明，最佳提取条件为料液质量体积比1.43、温度92℃、提取时间2.15 h。在此条件下，黑皮冬瓜多糖得率为2.33%。冬瓜多糖具有较好的抗氧化能力，3种自由基1,1-二苯基-2-苦基肼自由基（DPPH·）、羟基自由基、2,2,-联氮-双（3-乙基苯并噻唑啉-6-磺酸）二铵盐自由基（ABTS⁺·）的半数抑制浓度(IC₅₀)分别为6.71、1.91、3.04 mg/mL。冬瓜多糖具有与甘油基本相当的优异保湿性，还有一定的美白活性。更多还原

【Abstract】 Using black wax gourd（Benincasa hispida） as raw material, the extraction process for black wax gourd polysaccharides was optimized through single-factor and response surface experiments. The in vitro antioxidant activity, moisturizing and whitening activities of the obtained polysaccharides were evaluated. Results indicated that the optimal extraction conditions were the solid-to-liquid（mass-to-volume） ratio of 1.43, temperature of 92 ℃, and extraction time of 2.15 h. Under these conditions, the yield of black wax gourd polysaccharides reached 2.33%. The polysaccharides exhibited notable antioxidant capacity against 3 free radicals, the half maximal inhibitory concentration(IC₅₀) for 1, 1-diphenyl-2-picrylhydrazyl（DPPH·）, hydroxyl radical, and 2, 2,-bis（4-ethylbenzothiazoline-6-sulfonic acid） diammonium salt radical（ABTS⁺·）, were 6.71, 1.91, 3.04 mg/mL, respectively. Black wax gourd polysaccharides had good moisturizing properties, which was basically equivalent to the moisturizing properties of glycerol, and also possessed certain whitening activity.更多还原