【摘要】 研究木霉β-葡聚糖酶对辣椒幼苗的促生效果及对辣椒青枯病的生物防治作用，旨在为辣椒青枯病生物防治提供新思路。通过不同稀释倍数的β-葡聚糖酶液处理辣椒幼苗，30 d后检测分析辣椒各生长指标；采用人工接种病原菌的方法研究β-葡聚糖酶对盆栽辣椒青枯病的防控效果；分别于辣椒幼苗期、初花期、坐果期调查辣椒青枯病自然发病情况，探讨β-葡聚糖酶对辣椒青枯病的大田防治效果。结果显示，10倍稀释的β-葡聚糖酶溶液对辣椒幼苗茎粗、地上鲜质量和地下鲜质量的促生率均超过80%，显著提高了幼苗的健壮指数；β-葡聚糖酶液处理对辣椒青枯病的发生和流行有显著的抑制作用，在坐果期，不同稀释倍数β-葡聚糖酶液处理发病率比清水对照降低9.33～15.00个百分点，防效达42.11%～68.22%。木霉β-葡聚糖酶对辣椒幼苗有一定程度的促生作用，并显著提高其对青枯病的抗性，β-葡聚糖酶具有开发成生物制剂用于辣椒健身栽培和防控辣椒青枯病的前景。更多还原

【Abstract】 The purpose of this study is to investigate the growth promoting effect of β-glucanase from Trichoderma harzianum on pepper seedlings and its biological control effect on pepper wilt disease, in order to provide new reference directions for the biological disease control. Pepper seedlings were treated with different dilutions of β-glucanase solution, and after 30 days of transplantation, various growth indicators of peppers were detected and analyzed. Artificial inoculation of pathogenic bacteria was used to study the prevention and control effect of β-glucanase on potted pepper bacterial wilt disease. The incidence of pepper wilt disease during the seedling stage, initial flowering stage, and fruit setting stage was investigated, and the field control effect of β-glucanase on pepper wilt disease was explored. The results showed that the 10 fold solution of β-glucanase had a growth promoting rate of over 80% on the stem diameter, aboveground fresh mass, and underground fresh mass of pepper seedlings, and effectively improved the seedling strength index of pepper seedlings. The treatment of β-glucanase solution had a significant inhibitory effect on the occurrence and prevalence of pepper bacterial wilt. In the fruit setting stage of pepper, the incidence rate of β-glucanase solution treatment with different dilution ratios was 9.33%-15.00% lower than that of water control, and the control effect was 42.11%-68.22% respectively. β-glucanase from Trichoderma has a certain degree of growth promoting effect on pepper seedlings and significantly improves their resistance to bacterial wilt disease. β-glucanase has the potential to be developed into a biological agent for pepper fitness cultivation and prevention of pepper wilt disease.更多还原