【摘要】 背景：前期研究证实，补肾活血健脾方可提高骨质疏松症大鼠骨密度。目的：探索补肾健脾活血方对去卵巢大鼠骨质流失及骨小梁生物力学参数的影响。方法：将50只雌性SD大鼠随机分5组干预：正常组(n=10)不进行任何干预，假手术组(n=10)切除卵巢附近的脂肪组织，去势组(n=10)、补肾健脾活血方组(n=10)、阿仑膦酸钠组(n=10)切除双侧卵巢建立骨质疏松模型；造模12周后，补肾健脾活血方组、阿仑膦酸钠组分别灌胃给予补肾健脾活血方、阿仑膦酸钠，其他3组灌胃给予生理盐水，每天1次，连续给药12周。给药结束后，检测腰椎骨密度，micro-CT分析股骨远端及胫骨近端骨微结构参数，苏木精-伊红观察胫骨近端组织学形态，有限元分析股骨远端应变与应变。结果与结论：(1)去势组腰椎骨密度低于正常组、假手术组(P<0.05)，补肾健脾活血方组、阿仑膦酸钠组腰椎骨密度高于去势组(P<0.05)；(2)micro-CT分析显示，与正常组、假手术组比较，去势组胫骨近端(或股骨远端)骨体积分数、骨表面密度、骨小梁数量均降低(P<0.05)，骨小梁分离度、结构模型指数均升高(P<0.05)；与去势组比较，补肾健脾活血方组、阿仑膦酸钠组股骨远端的骨体积分数、骨表面密度、骨小梁数量均升高(P<0.05)，股骨远端的骨小梁分离度、结构模型指数均降低(P<0.05)，胫骨近端的骨体积分数、骨表面密度与骨小梁数量均升高(P<0.05)；(3)苏木精-伊红染色结果显示，去势组骨骼髓腔脂肪浸润程度高于正常组、假手术组，补肾健脾活血方组、阿仑膦酸钠组骨髓髓腔脂肪浸润程度明显得到改善；(4)有限元分析显示，相同载荷下，去势组骨小梁应力、应变高于正常组、假手术组(P<0.05)，补肾健脾活血方组、阿仑膦酸钠组骨小梁应力、应变低于去势组(P<0.05)。结果表明：补肾健脾活血方干预去卵巢大鼠可防止骨质流失、提升骨小梁生物力学性能，具有预防骨质疏松症骨折发生的潜在疗效。更多还原

【Abstract】 BACKGROUND:Previous studies have confirmed that the Bushen Jianpi Huoxue Formula can increase bone mineral density in osteoporotic rats.OBJECTIVE:To investigate the effects of the Bushen Jianpi Huoxue Formula on bone loss and trabecular biomechanical parameters in ovariectomized rats.METHODS:Fifty female Sprague-Dawley rats were randomly divided into five groups:a normal group(n=10) without any intervention,a sham-operated group(n=10) undergoing resection of adipose tissue near the ovaries,and an ovariectomized group(n=10),a Bushen Jianpi Huoxue Formula group(n=10),and an alendronate group(n=10) undergoing bilateral ovariectomy to establish an osteoporosis model.Twelve weeks after modeling,the Bushen Jianpi Huoxue Formula group and the alendronate group were orally administe red the Bushen Jianpi Huoxue Formula and alendronate,respectively,while the other three groups were orally administe red normal saline,once daily for 12 consecutive weeks.After final treatment,lumbar spine bone mineral density was measured,micro-CT was used to analyze bone microstructure parameters of the distal femur and proximal tibia,hematoxylin-eosin staining was used to observe the histological morphology of the proximal tibia,and finite element analysis was used to analyze strain and stress in the distal femur.RESULTS AND CONCLUSION:(1) Lumbar spine bone mineral density in the ovariectomized group was lower than that in the normal and sham-operated groups(P <0.05),while lumbar spine bone mineral density in the Bushen Jianpi Huoxue Formula group and the alendronate group was higher than that in the ovariectomized group(P<0.05).(2) Micro-CT analysis showed that compared with the normal and sham-operated groups,the ovariectomized group exhibited significantly decreased bone volume fraction,bone surface density,and trabecular number(P <0.05),as well as significantly increased trabecular separation and structure model index(P<0.05) in the proximal tibia(or distal femur).Compared with the ovariectomized group,the Bushen Jianpi Huoxue formula and alendronate groups showed significantly increased bone volume fraction,bone surface density,and trabecular number in the distal femur(P <0.05),significantly decreased trabecular separation and structure model index in the distal femur(P<0.05),and significantly increased bone volume fra ction,bone surface density,and trabecular number in the proximal tibia(P<0.05).(3) Hematoxylin-eosin staining results showed that the degree of fatty infiltration in the bone marrow cavity was significantly higher in the ovariectomized group than in the normal and sham-operated groups.The Bushen Jianpi Huoxue Formula and alendronate groups showed marked improvement in bone marrow adiposity.(4) Finite element analysis showed that under the same load,trabecular stress and strain in the ovariectomized group were significantly higher than those in the normal and sham-operated groups(P<0.05).The Bushen Jianpi Huoxue formula and alendronate groups exhibited significantly improved trabecular stress and strain compared with the ovariectomized group(P<0.05).In conclusion,the Bushen Jianpi Huoxue Formula can prevent bone loss,improve bone microstructure,and enhance trabecular biomechanical properties in ovariectomized rats,indicating its potential efficacy in preventing osteoporotic fractures.更多还原