【摘要】 通过叶片浸渍法和RT-qPCR技术，分析了3种不同杀螨机理的杀螨剂（阿维菌素、甲氰菊酯、炔螨特）对截形叶螨（Tetranychus truncatus）成螨的毒力及其对细胞色素P450基因表达的影响。毒力测定结果显示，阿维菌素、甲氰菊酯和炔螨特对截形叶螨的LC₅₀值分别为0.184 9、1.371 7和74.532 3 mg/L,毒力强度依次为阿维菌素>甲氰菊酯>炔螨特。基因表达分析表明，阿维菌素处理诱导6个P450基因（表达量上调1.61～15.63倍）显著上调；甲氰菊酯处理激活14个P450基因（表达量上调1.40～6.78倍）,其中 TtCYP392家族基因响应显著；炔螨特处理诱导15个P450基因（表达量上调1.54～18.51倍）表达升高。值得注意的是， TtCYP392D17和 TtCYP392E18能被3种杀螨剂共同诱导。研究结果表明，截形叶螨P450基因的表达模式与杀螨剂类型密切相关，揭示其在杀螨剂代谢中可能发挥重要作用，为解析叶螨抗药性机制提供了理论依据。更多还原

【Abstract】 This study investigated the toxicity of three acaricides with distinct modes of action(abamectin, fenpropathrin, and propargite) against adult Tetranychus truncatus and their effects on the expression of cytochrome P450 genes using the leaf-dipping method and RT-qPCR.Toxicity assays revealed that the LC50 values of abamectin, fenpropathrin, and propargite were 0.184 9,1.371 7,and 74.532 3 mg/L,respectively, with toxic potency ranked as abamectin> fenpropathrin> propargite.Gene expression analysis showed that abamectin treatment significantly upregulated six P450 genes(1.61-15.63-fold upregulation),while fenpropathrin activated 14 P450 genes(1.40-6.78-fold upregulation),with notable induction of TtCYP392 family genes, and propargite exposure activated 15 P450 genes(1.54-18.51-fold upregulation).Notably, TtCYP392D17 and TtCYP392E18 were co-induced by all three acaricides.These findings indicate that the expression patterns of P450 genes in T.truncatus were closely associated with acaricide types, suggesting their potential role in acaricide metabolism.This study provides a theoretical foundation for understanding the mechanisms of acaricide resistance in spider mites.更多还原