【摘要】 为证明基于RTILs的SEM预处理方法对湿软生物样品的有用性。本研究以RTILs简单滴加与吸干处理替代常规费时、繁琐及费用高的SEM预处理方法。结果表明，基于RTILs的SEM预处理法虽在保持样品形态方面不是非常完美，但与常规方法所得结果接近，且在孢子数量、孢子链、叶毛以及叶片表皮细胞等形态保持方面比常规SEM预处理法更接近于真实情况，且导电性能可媲美常规预处理法。所构建的基于RTILs的SEM预处理法在替代常规SEM预处理法方面存在巨大潜力，其具有经济、简便等特点，且所得图片质量与常规预处理法几乎一致，尤其在保持链格孢菌孢子串珠状形态、细胞饱满度等方面优于常规预处理法。更多还原

【Abstract】 To demonstrate the utility of the RTIL-based SEM pretreatment method for hydrated soft biological specimens,this study replaced the conventional time-consuming,labor-intensive,and expensive SEM sample preparation with a simple droplet application and blotting treatment.The results showed that although the RTIL-based method did not achieve perfect preservation of morphology,it produced results comparable to those of conventional methods.Specifically,it demonstrated superior structural fidelity in preserving spore quantity,spore chains,leaf trichomes,and epidermal cells,providing a more realistic representation of biological structures than traditional SEM pre-treatment.Additionally,its electrical conductivity was comparable to that of conventional methods.The developed RTIL-based approach shows great potential as an alternative to conventional SEM pretreatment,offering advantages in cost,simplicity,and efficiency,while maintaining image quality nearly identical to that of traditional protocols.Notably,it outperforms standard methods in preserving critical structural features such as the bead-like morphology of Alternaria spore chains and cellular turgor.更多还原