【摘要】 微生物的“暗物质”涵盖未被培养的微生物类群及其未知生物学特征，这一概念已被微生物学界广泛认可。随着微生物研究模式的不断发展，研究人员相继提出了一系列关于微生物的“暗物质”概念，且大量有关“暗物质”的研究进展已被报道。然而，这些概念内涵复杂且相互交叉，其内在联系与区别尚未得到清晰梳理。本文系统梳理了当前各类微生物相关“暗物质”的概念框架，并分析了微生物相关“暗物质”的研究方法及发展趋势。这将有助于微生物资源领域的研究者更清晰地认识微生物相关“暗物质”的内涵与研究现状，推动微生物的“暗物质”资源挖掘工作向更深层次迈进。更多还原

【Abstract】 The dark matter of microbes, encompassing uncultivated microbial taxa and unknown biological features, has been widely accepted by microbiologists. With the advancement of microbial investigation methods, a series of dark matter concepts related to microbes have been successively proposed, and numerous research advances in this field have been well-documented. However, these concepts exhibit complex and overlapping connotations, and their inherent connections and distinctions have not yet been clearly clarified. This review work systematically summarizes the current conceptual frameworks of different dark matter types of microbes and analyzes the relevant investigation methodologies and their development trends. This article will help researchers in the microbial resource field gain a clearer understanding of the conceptual connotation and current research status of microbe-associated dark matter and promote the in-depth development of dark matter resource mining.更多还原