【摘要】 以马铃薯（Solanum tuberosum L.）品种‘费乌瑞它’为材料，研究培养基类型、外源激素、碳源、活性炭及水质等多种因素对马铃薯脱毒苗组培快繁的影响。结果表明：马铃薯脱毒苗在MS培养基上的长势优于在B5和N6培养基上的长势，但在B5培养基中单株叶片数与新生芽苗数显著高于MS培养基的同类指标；添加1～2 g/L活性炭有利于马铃薯脱毒苗的生长与增殖，平均单株叶片数与新生芽苗数分别高于未添加活性炭处理组45.7%和47.7%；添加0.5 mg/L 6-BA与0.2 mg/L NAA可有效促进马铃薯脱毒苗的生长。用20～25 g/L白砂糖代替MS培养基中的蔗糖作为碳源，在制作培养基时使用自来水，不仅有利于提高脱毒苗的质量和繁殖效率，还可有效降低生产成本。更多还原

【Abstract】 Using potato cultivar ‘Favorita’ as the material, the effects of media type, exogenous hormone, carbon source, activated carbon and water quality on tissue culture and rapid propagation of virus-free potato seedlings were studied. The results showed that the growth of virus-free potato seedlings on MS medium was superior to that on B5 and N6 media. However, the number of leaves per plant and number of new shoots on B5 medium were significantly higher than those on MS. The addition 1-2 g/L activated carbon was beneficial to the growth and proliferation of virus-free potato seedlings, with the average number of leaves and new shoots being 45.7% and 47.7% higher than those of the control group without activated carbon respectively. The addition of 0.5 mg/L 6-BA and 0.2 mg/L NAA could effectively promote the growth of virus-free potato seedlings. Replacing sucrose in MS medium with 20-25 g/L white sugar as the carbon source, and using tap water in the preparation of medium, not only improved the quality and propagation efficiency of virus-free seedlings, but also effectively reduced the production costs.更多还原