【摘要】 [目的]前期研究中，课题组获得1株对马铃薯疮痂病具有显著抑菌活性和防治效果的贝莱斯芽孢杆菌Apcs-1。为进一步解析其生防作用机制。[方法]对该菌株Apcs-1进行全基因组测序分析，同时对施用生防菌后对土壤微生物菌群影响进行了测定。[结果]菌株Apcs-1基因组序列全长3 929 735 bp,GC含量46.50%，该菌株含有与抗生、促生诱导抗性等多种生防相关的功能基因；通过antiSMASH预测到菌株具有脂肽类、聚酮类、铁载体类等丰富的次级代谢产物合成基因簇，具备产生多种抗菌活性物质的潜力。VFDB数据库预测发现菌株具有防御性毒力因子、非特异性毒力因子、攻击性毒力因子、毒力相关调控基因。进一步对施用菌株发酵液处理后的根际土壤微生物群落分析表明，Apcs-1能够显著提升根际有益微生物(如放线菌门Actinobacteria、芽孢杆菌纲Bacilli等)的丰度与群落均匀度，并在土壤中显著富集氨基酸代谢、糖类代谢等关键代谢通路相关基因。[结论]从基因组和对微生物群落影响层面揭示了菌株Apcs-1的生防功能基础，为菌株在马铃薯疮痂病绿色防控中的开发应用提供了理论依据。更多还原

【Abstract】 [Objective]In previoaus studies, our research group isolated a strain of Apcs-1 with significant antagonistic activity against potato common scab. [Method]To further elucidate its biocontrol mechanism, whole-genome sequencing and analysis of strain Apcs-1 were performed in this study, and the effects of this biocontrol bacterium on soil microbial communities were also determined.[Results]The results showed that the complete genome of strain Apcs-1 is 3 929 735 bp in length with a GC content of 46.50%, and harbors multiple functional genes related to biocontrol traits such as antibiosis, growth promotion and induced resistance. AntiSMASH analysis predicted abundant biosynthetic gene clusters encoding lipopeptides, polyketides, siderophores and other secondary metabolites, indicating a strong potential to produce diverse antimicrobial substances. Prediction using the VFDB database revealed that the strain carries defensive virulence factors, nonspecific virulence factors, aggressive virulence factors, and virulence-related regulatory genes. Further analysis of rhizosphere soil microbial communities after application of the strain fermentation broth demonstrated that B. velezensis Apcs-1 significantly increased the abundance and community evenness of beneficial rhizosphere.microorganisms(e.g., phylum Actinobacteria, class Bacilli), and significantly enriched genes associated with key metabolic pathways including amino acid metabolism and carbohydrate metabolism in soil. [Conclusion]This study reveals the genetic basis and biocontrol functions of strain Apcs-1 at the genomic level and from the perspective of microbial community effects, providing a theoretical foundation for the development and application of this strain in the green control of potato common scab.更多还原