【摘要】 目的：研究LPS诱导牙周炎症对结合上皮附着功能的影响。方法：使用雄性野生型C57BL/6小鼠，上颌第一二磨牙腭侧局部注射E.coli-LPS，建立小鼠牙周炎模型，实时荧光定量PCR检测小鼠牙周组织Il-1β、Mmp-8、Mmp-9、Amtn、Odaph的mRNA表达，免疫组织化学分析小鼠牙周组织AMTN、ODAPH、TLR4的蛋白表达。结果：与对照组相比，牙周组织Il-1β、Mmp-8、Mmp-9mRNA表达不同程度上调（P<0.05）,Amtn在牙周组织中mRNA表达下调（P<0.05）。免疫组化显示，AMTN及ODAPH定位于结合上皮，二者表达强烈，随炎症不断发展，二者蛋白表达均显著下调，TLR4仅少量定位于结合上皮内基板偏冠方，随炎症发展TLR4蛋白表达上调。结论：结缔组织内LPS可能通过激活TLR4促使AMTN、ODAPH表达下调，引发结合上皮附着功能的破坏。更多还原

【Abstract】 Objective:To investigate the effects of periodontal inflammation induced by LPS on adhesion function of junctional epithelium.Methods:Male wild-type C57BL/6 mice were injected with E.coli-LPS in the palatine side of the maxillary first and second molar to establish mice periodontitis models.The mRNA expression of Il-1β,Mmp-8,Mmp-9,Amtn and Odaph in the maxillary molar region of the mice was detected by real-time PCR.While the protein expression of AMTN,ODAPH and TLR4 was analyzed by immunohistochemistry.Results:Compared with the control group,the mRNA expression levels of Il-Iβ,Mmp-8 and Mmp-9in maxillary molar region were up-regulated(P<0.05),and the mRNA expression of Amtn in periodontal tissue was down-regulated(P<0.05).Immunohistochemistry showed that AMTN and ODAPH were strongly expressed in the junctional epithelium,and were significantly down-regulated with the continuous development of inflammation.TLR4 was only slightly located in the basal lamina of the junctional epithelial crown.The expression of TLR4 protein increased with the development of periodontal inflammation.Conclusion:LPS induced periodontal inflammation may promote the activation of TLR4 followed by AMTN and ODAPH down-regulation and result in the failure of epithelial attachment.更多还原