【摘要】 目的 观察养卵方(YLF)对卵巢储备功能下降(DOR)大鼠卵巢组织SIRT3/FOXO3a和PLA2/COX2信号通路的影响，探讨YLF改善DOR卵巢储备功能的作用与机制。方法 将SD大鼠随机分为对照组和模型组，采用腹腔注射脱氧乙烯基环己烯(VCD)制备DOR模型大鼠，将造模成功的DOR大鼠随机分为模型组、YLF低剂量(YLF-L)组、YLF中剂量(YLF-M)组、YLF高剂量(YLF-H)组和补佳乐(BJL)组，各组分别予以相应药物灌胃，连续15 d。比较各组大鼠性腺(卵巢和子宫)指数，HE染色观察卵巢组织病理形态学变化，检测血清FSH、LH、AMH水平，Western blot检测卵巢组织SIRT3、FOXO3a、PLA2、COX2蛋白表达水平，PCR检测卵巢组织SIRT3、FOXO3a、PLA2、COX2、SOD、GSH-Px、CAT mRNA的表达水平。结果 与对照组比较，模型组卵巢和子宫指数下降(P<0.05),血清FSH、LH、FSH/LH升高(P<0.05),AMH水平降低(P<0.05),卵巢组织SIRT3、FOXO3a、PLA2、COX2蛋白和mRNA及SOD、GSH-Px、CAT mRNA表达水平降低(P<0.05);与模型组比较，中高剂量YLF干预后DOR大鼠性腺指数及卵巢病理形态改善，卵巢和子宫指数增加(P<0.05),血清FSH、LH、FSH/LH降低(P<0.05),AMH水平升高(P<0.05),卵巢组织SIRT3、FOXO3a、PLA2、COX2蛋白和mRNA及SOD、GSH-Px、CAT的mRNA表达均升高(P<0.05)。结论 YLF可能通过调控SIRT3/FOXO3a和PLA2/COX2信号通路，提高抗氧化酶活性，调节内分泌功能，缓解VCD诱导的卵巢损伤。更多还原

【Abstract】 Objective To observe the effects of YangLuanfang(YLF) on SIRT3/FOXO3-a and PLA2/COX2 signaling pathways in ovarian tissue of rats with diminished ovarian reserve(DOR), and explore the effect and mechanism of YLF on improving ovarian reserve function in rats with DOR. Methods SD rats were randomly divided into control group and model group. DOR model rats were made by intraperitoneal injection of vinylcyclohexene dioxide(VCD). The successful DOR rats were randomly divided into model group, YLF low-dose(YLF-L) group, YLF middle-dose(YLF-M) group, YLF high-dose(YLF-H) group and progynova(BJL) group. Each group was given corresponding drugs by gavage for 15 days. The indexes of gonads(ovaries and uterus) were compared in each group, and observe the pathological morphological changes of ovarian tissues by HE staining. Serum FSH, LH and AMH levels were detected. Western blot was used to detect the expression levels of SIRT3,FOXO3a, PLA2 and COX2 proteins in ovarian tissues. PCR was used to detect the mRNA expression levels of SIRT3, FOXO3a, PLA2, COX2, SOD,GSH-Px and CAT in ovarian tissue. Results Compared with the control group, the index of ovary and uterus in the model group decreased(P<0.05), the levels of serum FSH,LH and the FSH/LH ratio increased(P<0.05), AMH level decreased(P<0.05), and the expression levels of SIRT3, FOXO3a, PLA2, COX2 protein and mRNA, SOD, GSH-Px, and CAT mRNA in ovarian tissue decreased(P<0.05). Compared with the model group, the moderate to high doses of YLF improved the ovary pathological morphology of DOR rats, increased the ovarian and uterine index(P<0.05), decreased the levels of serum FSH, LH and the FSH/LH ratio(P<0.05), increased the AMH level(P<0.05), and increased the expressions of SIRT3, FOXO3a, PLA2, COX2 protein and mRNA, and the mRNA of SOD, GSH-Px and CAT in ovarian tissue(P<0.05). Conclusion YLF may improve the activity of antioxidant enzymes, regulate endocrine function, and alleviate ovarian damage induced by VCD by regulating SIRT3/FOXO3a and PLA2/COX2 signaling pathways.更多还原