【摘要】 以菊粉为基础碳源，筛选适宜的添加量，在此基础上复配添加灵芝多糖（Ganoderma lucidum polysaccharide,GLP），并与菊粉高添加量组（菊粉低添加量与灵芝多糖添加量之和）进行比较，通过测定菌体量(D₆₀₀)、总糖消耗量和短链脂肪酸（short chain fatty acids,SCFAs）含量，探究GLP对7株益生菌[加氏乳杆菌（Lactobacillus gasseri）、青春双歧杆菌（Bifidobacterium adolescentis）、丁酸梭菌（Clostridium butyricum）、脆弱拟杆菌（Bacteroides fragilis）、卵形拟杆菌（Bacteroides ovatus）、多形拟杆菌（Bacteroides thetaiotaomicron）和单形拟杆菌（Bacteroides uniformis）]生长及代谢的影响。结果表明：灵芝多糖均可被7株益生菌消耗利用，可显著促进加氏乳杆菌、丁酸梭菌、脆弱拟杆菌、卵形拟杆菌和单形拟杆菌生长；7株益生菌在灵芝多糖组的总SCFAs含量均显著高于低添加量菊粉组，且脆弱拟杆菌、单形拟杆菌在灵芝多糖组的总SCFAs含量还显著高于高添加量菊粉组(分别增加7.67、4.59 mmol·L^-1，相对提高58.61%、19.89%)。研究结果可为灵芝多糖作为靶向益生元制剂的开发提供参考。更多还原

【Abstract】 Using inulin as the basal carbon source,the optimal addition amount was screened for different species of probiotics.Based on the optimal inulin addition amount for each probiotic species,Ganoderma lucidum polysaccharide （GLP） was co-supplemented,and its effects were compared with a high inulin addition group,where the inulin addition amount equaled the sum of the optimal inulin addition amount from the screening experiments and the addition amount of GLP.By measuring bacterial biomass (D₆₀₀),total sugar consumption,and contents of shortchain fatty acids （SCFAs）,the effects of GLP on the growth and metabolism of seven probiotic strains （Lactobacillus gasseri,Bifidobacterium adolescentis,Clostridium butyricum,Bacteroides fragilis,B.ovatus,B.thetaiotaomicron and B.uniformis） were investigated.The results showed that GLP was consumed and utilized by all seven probiotic species.It significantly promoted the growth of L.gasseri,C.butyricum,B.fragilis,B.ovatus and B.uniformis.The total SCFAs contents in the GLP groups for all seven probiotic species were significantly higher than those in the optimal inulin addition groups.Furthermore,the total SCFAs contents in the GLP groups of B.fragilis and B.uniformis were also significantly higher than those in their high inulin groups (increased by 7.67 and 4.59 mmol·L^-1,respectively,corresponding to relative increases of 58.61%and 19.89%).These findings provided a reference for the development of GLP as a targeted prebiotic preparation.更多还原