【摘要】 自河北省承德市围场县塞罕坝采集木蹄层孔菌(Fomes fomentarius)野生子实体，组织分离获得菌株后进行鉴定，采用单因素实验和正交实验研究菌株的生物学特性，并驯化。结果表明：最适碳源、氮源、温度、pH分别为果糖、酵母浸粉、28℃、7，正交实验确定的最佳组合是果糖、酵母浸粉、28℃、pH6；菌丝50～55 d长满栽培袋，40～45 d后形成半球形原基，25～35 d后可采收，子实体直径4.5～6.4 cm、基部厚2.5～3.0 mm。研究结果可为木蹄层孔菌开发利用提供参考。更多还原

【Abstract】 Wild fruiting bodies of Fomes fomentarius were collected from Betula platyphylla in Saihanba Mechanised Tree Farm, Weichang County, Chengde City, Hebei Province.After tissue isolation, a strain was identified as F.fomentarius, and then the strain was studied for biological characteristics through single-factor experiments and orthogonal experiments.Finally, the strain was domesticated.The results showed that the optimal carbon source, nitrogen source, temperature, and initial pH in single-factor experiments were fructose, yeast extract powder, 28 ℃, and pH7, respectively.The optimal conditions determined by the orthogonal experiments were fructose, yeast extract powder, 28 ℃, and pH6.After inoculation, the mycelia of the isolated F.fomentarius strain fully colonized the cultivation bags within 50–55 d, and hemispherical primordia formed after an additional 40–45 d.Harvesting was possible after a further 25–35 d of cultivation, with the fruiting bodies measuring 4.5–6.4 cm in diameter and having a base thickness of 2.5–3.0 mm.These findings provided a reference for subsequent development and utilization of F.fomentarius.更多还原