【摘要】 目的:研究分析茯苓多糖(PCP)调控SREBP1信号通路对非酒精性脂肪肝(NAFLD)大鼠肝脏脂质沉积的影响。方法:SFP雄性SD大鼠60只，随机分为对照组、模型组、低剂量组、高剂量组(100、300 mg/kg),喂养6周高脂饲料建立NAFLD模型。模型组灌胃蒸馏水，低剂量组、高剂量组大鼠灌胃不同剂量PCP,连续给药8周，处死并取出大鼠肝脏组织，体外进行HepG2细胞培养，游离脂肪酸诱导NAFLD细胞模型，低剂量组和高剂量组加入不同浓度的茯苓多糖培养24 h。检测各组大鼠血脂、抗氧化应激指标，行细胞油红O染色。荧光定量PCR检测各组大鼠肝组织中固醇调节元件结合蛋白1(SREBP1)、脂肪酸合酶(FASN)、硬脂酰辅酶A去饱和酶1(SCD1)的表达。结果:与对照组比较，模型组总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、丙二醛(MDA)表达水平升高(均P<0.05),模型组HDL-C、SOD、GSH-Px水平、SREBP1信号通路相关蛋白表达降低(均P<0.05)。与模型组、低剂量组比较，高剂量组TG、TC、LDL-C、MDA水平下降，HDL-C、SOD、GSH-Px水平升高(均P<0.05)。油红O染色后，模型组比对照组细胞内红色脂滴的数量增多，与模型组比较，低剂量组、高剂量组细胞内红色脂滴数量减少(P<0.05)。结论:茯苓多糖能够降低肝脏内脂质积累，改善血脂水平，可有效改善体内氧化应激反应，其作用机制为调节SREBP1脂质合成相关信号通路。更多还原

【Abstract】 Objective:To investigate and analyze the effect of Poria cocos polysaccharides(PCP) regulating the SREBP1 signaling pathway on hepatic lipid deposition in non-alcoholic fatty liver disease(NAFLD) rats.Methods:A high-fat diet was used to establish a NAFLD rat model in 60 rats, randomly divided into control group, model group, low and high dose groups.Establish a NAFLD model with high-fat diet and feed it for 6 weeks.The model group was given distilled water by gavage, while the low-dose and high-dose groups of rats were given different doses of PCP by gavage for 8 consecutive weeks.Rats were euthanized and their liver tissues were taken out for HepG2 cell culture in vitro.Free fatty acid induced NAFLD cell models were established, and the low-dose and high-dose groups were cultured in different concentrations of Poria cocos polysaccharides for 24 hours.Blood lipids and antioxidant stress indicators were detected in each group of rats, and cell oil red O staining was performed.Fluorescence quantitative PCR was used to detect the expression of SREBP1,FASN,and SCD1 in liver tissues of each group.Results:Compared with the control group, the expression levels of TC,TG,LDL-C,MDA in the model group were increased(all P<0.05),while the levels of HDL-C,SOD,GSH-Px, and SREBP1 signaling in the model group were decreased(all P<0.05).Compared with the model group and the low-dose group, the levels of TG,TC,LDL-C,and MDA in the high-dose group were decreased, and the levels of HDL-C,SOD,and GSH-Px were increased(all P<0.05).After Oil Red O staining, the number of red lipid droplets in the cells of the model group was larger than that in the control group.Compared with the model group, the number of red lipid droplets in the cells of the low-dose group and the high-dose group was decreased(P<0.05).Conclusion:Poria cocos polysaccharides can reduce lipid accumulation in the liver, improve blood lipid levels, and effectively improve oxidative stress response in the body.Its mechanism of action is to regulate the SREBP1 lipid synthesis related signaling pathway.更多还原