【摘要】 为实现三文鱼新鲜度的快速、无损检测，解决水产品新鲜度检测效率低、易损伤样品的技术难题，本研究采用原位生长法将全无机钙钛矿量子点封装于UiO-66金属有机框架中，构建了新型CsPbBr₃@UiO-66复合荧光探针，该探针在室温下对氨气具有快速、灵敏的荧光响应。采用场发射透射电子显微镜等表征技术，系统分析了CsPbBr₃@UiO-66复合荧光探针的微观结构；同时探讨了该复合探针对NH₃的响应机理。结果表明：CsPbBr₃@UiO-66与NH₃之间产生静态猝灭效应，可实现对NH₃的特异性识别。该复合荧光探针可在120 s内完成对氨气的有效响应，检测限低至2.07 ppm,且表现出良好的稳定性和选择性，可有效排除其他干扰物质的影响。将该探针应用于25℃贮藏条件下三文鱼的新鲜度监测，结果显示，探针荧光强度的变化与三文鱼中挥发性盐基氮（TVB-N）含量呈现良好的线性关系，证实该复合荧光探针技术可有效应用于水产品中挥发性胺类物质的快速、无损检测，为三文鱼等水产品新鲜度的现场快速检测提供了技术支撑。更多还原

【Abstract】 To enable rapid, non-destructive detection of salmon freshness and address the technical challenges of low efficiency and sample damage in aquatic product freshness assessment, a novel CsPbBr₃@UiO-66 composite fluorescent probe was developed by encapsulating all-inorganic perovskite quantum dots into the UiO-66 metal-organic framework via in situ growth method. The probe exhibited rapid and sensitive fluorescence response to ammonia gas（NH₃） at room temperature. The microstructure of the CsPbBr₃@UiO-66 composite fluorescent probe was systematically characterized using techniques such as field-emission transmission electron microscopy. Furthermore, the response mechanism of CsPbBr₃@UiO-66 to NH3 was investigated. The results indicated that a static quenching effect occurs between CsPbBr₃@UiO-66 and ammonia, enabling specific recognition of ammonia. Experimental results demonstrated that the composite probe achieves effective response to NH3 within 120 s, with a limit of detection as low as 2.07 ppm, while exhibiting excellent stability and selectivity. The probe applied to monitor the freshness of salmon stored at 25 ℃ showed a strong linear correlation between its fluorescence intensity changes and the total volatile basic nitrogen（TVB-N） content in salmon. The results confirmed that this composite fluorescent probe technology can be effectively used for rapid, non-destructive detection of volatile amines in aquatic products. This study provides technical support for on-site rapid freshness detection of salmon and other aquatic products.更多还原