【摘要】 为探究生物炭对重金属和微塑料复合污染下苦荞根际微生态的影响，通过盆栽试验分析了在铅(Pb)和聚乳酸微塑料(PLA,polylactic acid)复合污染下，添加生物炭后苦荞农艺性状、土壤理化性质及根际细菌群落的变化。结果表明：与污染处理(C3)相比，生物炭使苦荞株高和根长分别增长了45.0%和76.7%;根鲜重增加55.5%,根干重增加40%;茎鲜重增加76.6%,茎干重增加14.1%;土壤提高pH值0.54个单位，土壤全氮增加0.09 g/kg、碱解氮增加0.64 mg/kg、有效磷增加6.68 mg/kg、速效钾增加46.34 mg/kg,处理间的显著性差异P <0.05。同时，生物炭优化了根际细菌群落结构，使土壤优势菌门变形菌门(Proteobacteria)相对丰度降低1.36%、放线菌门(Actinobacteriota)相对丰度增加4.19%、绿弯菌门(Chloroflexi)相对丰度降低1.75%,优势菌属鞘氨醇单胞菌属(Sphingomonas)相对丰度增加3.38%、芽孢杆菌属(Gemmatimonas)相对丰度增加0.45%、细菌LWQ8相对丰度增加3.00%。冗余分析(RDA)表明，有效磷、pH和速效钾是驱动根际微生物群落组成变化的关键环境因子。研究证实，生物炭通过改善土壤理化性质和重塑有益微生物群落，缓解了Pb和PLA对苦荞的胁迫，促进了植株生长和根际微生态的稳定。该结果可为重金属-微塑料复合污染土壤的修复提供理论依据和技术参考。更多还原

【Abstract】 In order to explore the effect of biochar on the microecology of Tartary Buckwheat Rhizosphere under the combined pollution of heavy metals and micro plastics, a pot experiment was conducted to analyze the changes of agronomic properties, soil physical and chemical properties and rhizosphere bacterial community of Tartary Buckwheat after the biochar was added under the combined pollutant of lead(PB) and polylactic acid(PLA). The results showed that compared with the pollution treatment(C3), the plant height and root length of Tartary Buckwheat increased by 45.0% and 76.7%, respectively; the fresh weight and dry weight of roots increased by 55.5% and 40%, respectively; the fresh weight and dry weight of stems increased by 76.6% and 14.1%, respectively; andthe soil pH value increased by 0.54 unit, the total nitrogen increased by 0.09 g/kg, the alkali hydrolyzable nitrogen increased by 0.64 mg/kg, the available phosphorus increased by 6.68 mg/kg, and the available potassium increased by 46.34 mg/kg, with significant differences between the treatments(P<0.05). Additionally, biochar optimized the rhizosphere bacterial community structure, which reduced the relative abundance of Proteobacteria, Actinobacteria, Chloroflexi, Sphingomonas, Gemmatimonas and LWQ8 by 1.36%, 4.19%, 1.75%, 3.38%, 0.45% and 3.00%, respectively. Redundancy analysis(RDA) showed that available phosphorus, pH and available potassium were the key environmental factors driving the change of rhizosphere microbial community composition. The study confirmed that biochar alleviated the stress of Pb and PLA on Tartary Buckwheat by improving soil physical and chemical properties and remodeling beneficial microbial community, and promoted plant growth and the stability of rhizosphere microecology. The results can provide theoretical basis and technical reference for the remediation of heavy metal micro plastic composite contaminated soil.更多还原