【摘要】 为提高植物乳植杆菌耐胁迫能力，文章采用内源乳化法和挤压法，以植物乳植杆菌作为芯材、海藻酸钠和刺梨多糖作为复合壁材制备微胶囊晶球。通过单因素、响应面优化试验，以包埋率为评价指标，确定微胶囊晶球最佳制备工艺条件为水油体积比1∶3、芯壁质量比1∶3、海藻酸钠质量分数3%，该条件制备的植物乳植杆菌微胶囊晶球包埋率为86.37%。进一步测定微胶囊晶球水分含量、质构特性、傅里叶红外光谱、热稳定性及体外模拟肠溶液释放活菌数。结果表明，植物乳植杆菌微胶囊晶球含水量为76.94%、硬度为828.13 g，结构完整，热稳定性良好，在体外模拟肠溶液中所释放的游离活菌数随时间的延长而增加，在60 min时活菌数释放量达到2.38×10~9 CFU/g，试验制备的植物乳植杆菌微胶囊晶球具有良好的理化稳定性。更多还原

【Abstract】 In order to improve the stress resistance of Lactiplantibacillus plantarum, the microcapsule crystal spheres were prepared with Lactiplantibacillus plantarum as core material, sodium alginate and polysaccharide of Roxanthus as composite wall material by internal emulsification and extrusion method. Through the single factor and response surface optimization test, the embedding rate was used as the evaluation index, and the optimum preparation conditions were determined as the water-oil volume ratio of 1:3, the core wall mass ratio of 1:3 and the mass fraction of sodium alginate of 3%. The embedding rate of Lactiplantibacillus plantarum microcapsules was 86.37%. The moisture content, texture properties, Fourier infrared spectroscopy, thermal stability and the number of viable bacteria released from simulated intestinal solution in vitro were further determined. The results showed that the water content of Lactiplantibacillus plantarum microcapsules was 76.94%, the hardness was 828.13 g, and the structure was complete and the thermal stability was good. The number of free viable bacteria released in the simulated intestinal solution in vitro increased with the extension of time, and the release of viable bacteria reached 2.38×10~9 CFU/g at 60 min. In summary, the Lactiplantibacillus plantarum microcapsules prepared in this study have good physicochemical stability.更多还原