【摘要】 采用水热法合成苯丙氨酸模板化铜纳米簇（Phe-CuNCs），通过Phe-CuNCs与罗丹明6G（Rh6G）之间的静电相互作用，构建双发射比率荧光探针。该探针在310 nm激发下，于420 nm和553 nm处出现两个分离良好的发射峰，可在pH=7.0条件下实现人尿液样品中左氧氟沙星（LEV）浓度的测定。为探索Phe-CuNCs/Rh6G对LEV的选择性，除LEV外，还包括3种抗生素的荧光研究，分别为阿奇霉素（AZ）、红霉素（ER）、头孢泊肟（CD），它们与LEV具有结构相似性。LEV浓度在0～5??mol/L范围内连续添加目标物后，得回归方程F₁/F₂=0.010 4C_LEV+1.90，以信噪比S/N=3计算，检测限为0.03μmol/L。在人尿液样品中的实际应用结果显示，该探针的回收率为84.00%～102.00%，具备良好的实用性。更多还原

【Abstract】 Phenylalanine-templated copper nanoclusters（Phe-CuNCs） were synthesized via a hydrothermal method. A dual-emission ratiometric fluorescent probe was constructed based on the electrostatic interaction between Phe-CuNCs and Rhodamine 6G（Rh6G）. Under the excitation wavelength of 310 nm, the probe exhibited two well-resolved emission peaks at 420 nm and 553 nm, which could be applied to the determination of levofloxacin（LEV） in human urine samples at pH=7.0. To explore the selectivity of Phe-CuNCs/Rh6G toward LEV, fluorescence investigations were also conducted on three other antibiotics besides LEV, namely azithromycin（AZ）, erythromycin（ER） and cefpodoxime（CD）. With the continuous addition of LEV within the concentration range of 0-5 ??mol/L, the regression equation was fitted as F₁/F₂=0.010 4C_LEV+1.90. The limit of detection was calculated to be 0.03 ??mol/L at a signal-to-noise ratio of S/N=3. The practical application in human urine samples presented satisfactory recoveries ranging from 84.00% to 102.00%, demonstrating favorable practical applicability of the probe.更多还原