【摘要】 目的 基于液相色谱-串联质谱(LC-MS/MS)检测方法，建立广西壮族自治区成年男性11氧雄激素参考值范围，为临床应用提供参考。方法 对中国精准队列(2017年1月至2019年12月)中的男性群体进行年龄分层随机抽样。最终纳入419例18～60岁男性，其中18～30岁143例、31～45岁170例、46～60岁106例。调取其体检资料和血清样本，用LC-MS/MS技术检测8种肾上腺皮质类固醇激素，分别是皮质酮、可的松、皮质醇、18-羟基皮质醇、雄烯二酮(A4)、11-羟基雄烯二酮(11-OH-A4)、脱氢表雄酮(DHEA)、脱氢表雄酮硫酸盐。分析11氧雄激素水平特征，及其与其他类固醇激素及血压、血糖、血脂等代谢指标的相关性。结果 成年男性群体中血清皮质酮、A4、DHEA、脱氢表雄酮硫酸盐的激素水平与年龄呈负相关(P<0.001)，但是11-OH-A4及可的松、皮质醇、18-羟基皮质醇等激素水平不随年龄变化; 8种皮质类固醇激素相互关联，但与BMI及血清胆固醇、高密度脂蛋白、低密度脂蛋白等脂代谢指标无显著相关性。最后，推荐18～60岁广西壮族自治区男性血清11-OH-A4参考值范围是3.71～6.50 nmol/L。结论 18～60岁男性11-OH-A4血清水平与年龄变化无显著相关性。建立11-OH-A4的LC-MS/MS检测方法，确定成年男性血清水平参考值范围，有助于11氧雄激素在临床的转化应用。更多还原

【Abstract】 Objective This study aims to establish a reference interval for 11-oxygenated androgens in Guangxi males using an liquid chromatography-tandem mass spectrometry(LC-MS/MS)-based detection method,which provides a reference for clinical practice. Methods A stratified random sampling by age group was performed on males from the China Precision Cohort with the physical examination data and serum samples being retrieved. A total of 419 male participants aged from 18 to 60 years were included and divided into three age groups: including 143 cases aged 18-30 years,170 cases aged 31-45 years,and 106 cases aged 46-60 years.Eight adrenal corticosteroids,including corticosterone,cortisone,cortisol,18-hydroxycortisol,androstenedione,11β-hydroxy androstenedione,dehydroepiandrosterone and dehydroepiandrosterone sulfate were quantified using LC-MS/MS. The characteristics of 11-oxandrolone levels,as well as their correlations with other steroid hormones and metabolic indicators such as blood pressure,blood sugar,and blood lipids were analyzed. Results The serum levels of Corticosterone,A4,DHEA and DHEAS exhibited a significant negative correlation with age(P < 0. 001),while levels of 11-OH-A4,cortisone,cortisol and 18-OH-cortisol remained unchanged across age groups. The eight corticosteroid hormones demonstrated significant intercorrelations,but no significant association was observed with BMI or serum lipid parameters,including total cholesterol,HDL and LDL. The serum reference interval for 11-OH-A4 in adult males aged from 18 to 60 years in Guangxi Zhuang Autonomous Region was recommended at 3. 71-6. 50 nmol/L. Conclusion Adrenal corticosteroids such as corticosterone and DHEA decrease with age,while adrenal 11-OH-A4 levels remain stable. Establishing the reference interval for serum 11-OH-A4 in adult males holds significant reference value for its clinical translation and application.更多还原