【摘要】 目的 研究人脐带间充质干细胞(h UCMSC)治疗慢性非细菌性前列腺炎(CNP)模型大鼠的有效性。方法 32只雄性SD大鼠随机分为对照组、模型组、坦索罗辛组和h UCMSC组，每组8只。除对照组外，其余各组大鼠构建CNP模型。28 d造模完成后，h UCMSC组每周尾静脉注射1×10~6个/kg P5代h UCMSC细胞悬液，连续4周;对照组和模型组注射等体积0.9%Na Cl溶液;坦索罗辛组每日灌胃0.022 mg/kg坦索罗辛溶液，持续28 d。治疗4周后，评估各组大鼠体重、摄食饮水量、前列腺指数等基础指标，利用HE染色观察前列腺组织病理形态，ELISA检测大鼠血清TNF-α、IL-1β、IL-10水平，RT-qPCR检测前列腺组织TNF-α、IL-1β mRNA表达，Western印迹检测NF-κB炎症通路蛋白表达。结果 治疗结束后，与模型组相比，坦索罗辛组和h UCMSC组大鼠体重和饮水量有所增加(P<0.05);前列腺指数和前列腺湿重显著降低(P<0.01);前列腺组织间质炎性细胞减少;血清TNF-α、IL-1β水平及前列腺组织TNF-α、IL-1βmRNA表达水平显著降低(P<0.01),IL-10水平升高(P<0.01); p-IκBα/IκBα、p-NF-κB p65/NF-κB p65比值显著减小(P<0.01)。结论 h UCMSC能改善大鼠前列腺病理状态，抑制促炎因子过表达，下调NF-κB信号通路磷酸化水平，缓解CNP大鼠的炎症反应。更多还原

【Abstract】 Objective To investigate the efficacy of human umbilical cord mesenchymal stem cell( h UCMSC) for the treatment of chronic nonbacterial prostatitis( CNP) model rats. Methods Thirty-two male Sprague-Dawley rats were randomized into four experimental subgroups: control,model,tamsulosin,and h UCMSC,with eight animals allocated to each group.CNP models were induced in all subgroups,except the control. After 28 days of modeling,the h UCMSC group was injected weekly with 1 × 10~6 cells/kg P5 h UCMSC cell suspension by tail vein for 4 weeks. In the control and model groups,equivalent volumes of normal saline were administered via injection. And the tamsulosin group was given daily gavage of 0. 022 mg/kg tamsulosin solution for 28 days. After 4 weeks of treatment,the basic indexes including body weight,intake and water intake,and prostate index were analyzed in each group,H&E staining was performed to characterize the histopathological features of the prostate. The levels of serum TNF-α,IL-1β,and IL-10 in rats were detected by ELISA. qRT-PCR was used to detect the mRNA expression of TNF-α and IL-1β in prostate tissues. And Western blot was performed to detect the protein expression of the NF-κB inflammatory pathway. Results At the end of treatment,compared with the model group,the body weight and water intake of the rats in the tamsulosin group and h UCMSC group increased significantly( P < 0. 05). There was a significant decline in prostate indexes and prostate wet weight( P < 0. 01). And interstitial inflammatory cells in prostate tissues were reduced. The levels of serum TNF-α and IL-1β and the expression of TNF-α and IL-1β mRNA in prostate tissues were significantly reduced( P <0. 01). An opposite tendency was observed in IL-10 levels( P < 0. 01). p-IκBα/IκBα and p-NF-κB p65/NF-κB p65 ratios were significantly reduced( P < 0. 01). Conclusion h UCMSC improve the pathological state of rat prostate,inhibit the overexpression of pro-inflammatory factors,down-regulate the phosphorylation level of NF-κB signaling pathway,and alleviate the inflammatory response in CNP rats.更多还原