【摘要】 为明晰黄淮麦区新育小麦品种（系）低分子量麦谷蛋白亚基（low molecular weight-glutenin subunits,LMW-GS）与籽粒品质的遗传多样性，以近年新审定或正在参试的84份小麦新品种（系）为研究对象，采用22对特异性引物对LMW-GS基因进行检测，同时结合蛋白质含量等6项籽粒品质指标进行综合分析，探索LMW-GS与品质的关系。结果表明，共检测到9种LMW-GS等位基因，其中Glu-A3位点检出A3d(58.33%)、A3a/c(47.62%)和A3a(4.76%)3种类型；Glu-B3位点仅检出B3d，频率为29.76%;Glu-D3位点检出D3-21(69.05%)、D3-32(58.33%)、D3-23(41.67%)、D3-31(2.38%)和D3-22(1.19%)5种类型。A3a/c和B3d与湿面筋含量呈显著正相关。LMW-GS组合类型共40种，频率较高的为A3d+D3-21(17.86%)、A3d+D3-23+D3-32(7.14%)和A3a/c+D3-21(7.14%)。在优质小麦品种中，Glu-B3d和Glu-D3-32的频率较高。依据籽粒品质指标将84份品种（系）划分为4个类群，其中类群Ⅱ在6项品质指标上表现较好；类群Ⅳ聚集了新麦26、西农979、郑麦366等强筋或中强筋的品种（系）。研究结果为小麦品质育种工作提供了重要的理论参考。更多还原

【Abstract】 To clarify the grain quality and genetic diversity of low-molecular-weight glutenin subunits(LMW-GS) in newly bred wheat varieties(lines) at the Huanghuai wheat region, 84 newly approved or under trial wheat varieties(lines) were used as materials. The composition and frequency distribution of LMW-GS were detected using 22 pairs of specific primers, and cluster analysis was conducted in combination with 6 grain quality indicators. The results showed that a total of 9 LMW-GS alleles were detected. At the Glu-A3 locus, 3 alleles were detected including GluA3d(58.33%), Glu-A3a/c(47.62%) and Glu-A3a(4.76%). At the Glu-B3 locus, only the Glu-B3d allele was detected, with a frequency of 29.76%. At the Glu-D3 locus, Glu-D3-21(69.05%), D3-32(58.33%), D3-23(41.67%), D3-31(2.38%) and D3-22(1.19%) alleles were detected. Both A3a/c and B3d showed a significant positive correlation with wet gluten content. A total of 40 LMW-GS combination types were identified, and the freqnencies of A3d+D3-21(17.86%), A3d+D3-23+D3-32(7.14%) combinations were high. Based on grain quality traits, 84 varieties(lines) were clustered into 4 groups. Group Ⅱ exhibited superior comprehensive quality characteristics. Group Ⅳ included multiple strong-gluten or medium-strong-gluten varieties, such as Xinmai 26, Xinong 979 and Zhengmai 366, etc. Above results provided important theoretical reference for wheat quality breeding.更多还原