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不同地区清香大曲微生物群落与理化指标关联分析

Correlation Analysis Between Microbial Community and Physical and Chemical Indexes of Qingxiang Daqu in Different Regions

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【作者】 孙威朱君卓曾月毛文定冯文聪陈晖廖蓓方尚玲陈茂彬

【Author】 SUN Wei;ZHU Junzhuo;ZENG Yue;MAO Wending;FENG Wencong;CHEN Hui;LIAO Bei;FANG Shangling;CHEN Maobin;Cooperative Innovation Center of Industrial Fermentation (Provincial-Ministerial Joint Construction),Key Laboratory of Fermentation Engineering (Ministry of Education),Hubei Key Laboratory of Industrial Microbiology,College of Life Sciences and Health Engineering,Hubei University of Technology Wuhan;Hubei Angel Yeast Co.,Ltd.;

【通讯作者】 陈茂彬;

【机构】 工业发酵省部共建协同创新中心,发酵工程教育部重点实验室,工业微生物湖北省重点实验室,湖北工业大学生命科学与健康工程学院湖北安琪酵母股份有限公司

【摘要】 该研究对3种不同地区清香型大曲(山西地区FJ、青海地区QK、湖北地区HL)进行理化指标分析,利用高通量测序技术分析微生物群落组成结构的差异,并对二者进行关联分析。结果表明曲样FJ的糖化力和液化力最高;曲样QK的酯化力和发酵力最高;曲样HL的糖化力和液化力最低;3种不同地区酒曲的发酵力差距较小。复膜孢酵母属(Saccharomycopsis)为曲样FJ和HL的优势真菌属;威克汉姆酵母属(Wickerhamomyces)为曲样QK优势真菌属。曲样FJ的优势细菌属为葡萄球菌属(Staphylococcus);曲样HL的优势细菌属为克氏杆菌属(Kroppenstedtia)、糖多孢菌属(Saccharopolyspora)和芽孢杆菌属(Bacillus);曲样QK的细菌属水平结构分布较为平均。通过对理化指标与优势菌属进行关联性分析,曲样中优势真菌属中曲青霉属(Penicillium)与大曲酯化力之间具有极显著正相关性。优势细菌属中明串珠球菌属(Leuconostoc)与样品糖化力之间具有正相关性;泛菌属(Pantoea)、明串珠球菌属(Leuconostoc)与样品大曲液化力之间具有显著正相关性;鞘氨醇单胞菌属(Sphingomonas)与样品酯化力具有显著正相关性。

【Abstract】 This study analyzed the physicochemical properties and microbial community structures of three Qingxiang-type daqu samples(FJ from Shanxi, QK from Qinghai, and HL from Hubei) using high-throughput sequencing. Results showed that FJ had the highest saccharifying and liquefying activities, QK had the highest esterifying and fermenting activities, while HL had the lowest saccharifying and liquefying activities. The fermentative power differences among the three samples were small. Saccharomycopsis was the dominant fungal genus in FJ and HL, while Wickerhamomyces was dominant in QK. Staphylococcus was the dominant bacterial genus in FJ, while Kroppenstedtia, Saccharopolyspora, and Bacillus were dominant in HL. QK had a more evenly distributed bacterial community. Correlation analysis indicated that Penicillium had a significant positive correlation with esterifying activity, Leuconostoc had a positive correlation with saccharifying activity, and Pantoea, Leuconostoc, and Sphingomonas had significant positive correlations with liquefying, esterifying activities respectively.

【基金】 “十三五”国家重点研发计划重点专项(2016YFD0400500);湖北省科技厅重大专项(2018ABA084)
  • 【分类号】TS261.11
  • 【下载频次】162
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