【摘要】 目的 比较白光LED、红光LED以及荧光灯为光源的亚甲蓝光化学法对血浆病毒灭活效果。方法 血浆中加入终浓度为1??μmol/L的亚甲蓝，分别用白光LED、红光LED和荧光灯照射30??min，比较处理后血浆中凝血因子（纤维蛋白原[FIB]、因子[F]Ⅱ、FⅤ、FⅦ、FⅧ、FⅨ、FⅩ、FⅪ、FⅫ、血管性假血友病因子抗原[vWF:Ag]、血管性假血友病因子活性[vWF:Ac]）、抗凝血蛋白（抗凝血酶[AT]、蛋白C[PC]）的活性、凝血酶原时间（PT）和部分活化凝血酶原时间（APTT），以及对血浆中Sindbis病毒的灭活效果。结果 三种光源的亚甲蓝光化学法处理都会使血浆中凝血因子和抗凝血蛋白活性受损，凝血时间延长，其中FⅤ、FⅦ、FⅨ和FⅪ、vWF:Ac和AT的活性变化在三种光源间没有显著性差异（P=0.115??8，0.434??2，0.610??8，0.094??3，0.137??7，0.166??7），PT、APTT的凝血时间变化也没有显著性差异（P=0.652??6，0.138??2）。红光LED的FIB、FⅧ、FⅫ和PC低于白光LED（P<0.000??1，P<0.000??1，P=0.023??2，P<0.000??1）和荧光灯（P<0.000??1，P<0.000??1，P=0.003??4，P<0.000??1）。荧光灯的FⅡ高于红光LED（P=0.012??2），FⅩ高于白光LED和红光LED（P=0.036??6，0.006??8）。白光LED的vWF:Ag显著高于红光LED和荧光灯（P=0.014??3，0.017??2）。经过三种光源的亚甲蓝光化学处理，血浆中的Sindbis病毒效价下降均>4 LogTCID₅₀/0.1??mL。结论 三种光源的亚甲蓝光化学法处理都能有效灭活血浆中的Sindbis，三种光源中红光LED对血浆中FIB和FⅧ活性影响最大，白光LED可作为未来血浆病毒灭活处理的首选光源。更多还原

【Abstract】 Objective To compare the viral inactivation effects of methylene blue（MB） plus light （MB/light） treatment using white light LEDs,red light LEDs,and fluorescent lamps on plasma.Methods Plasma samples were treated with methylene blue at a final concentration of 1 μM,followed by 30??minutes of irradiation using white light LEDs,red light LEDs,or fluorescent lamps.The study evaluated the impact of these treatments on coagulation factors （FIB,FⅡ,FⅤ,FⅦ,FⅧ、FⅨ,FⅩ,FⅪ,FⅫ,vWF:Ag,vWF:Ac）,anticoagulant proteins （AT,PC）,PT,and APTT.Additionally,the efficacy of SINDBIS virus inactivation in plasma was assessed.Results All three methylene blue photodynamic therapies significantly reduced the activity of coagulation factors and anticoagulant proteins,and prolonged coagulation times.No significant differences were found in the activity of FⅤ,FⅦ,FⅨ,FⅪ,vWF:Ac,and AT among the three light sources （P=0.115??8,0.434??2,0.610??8,0.094??3,0.137??7,0.166??7）.There were no significant differences in the changes of PT and APTT （P=0.652??6,0.138??2）.Red light LEDs treatment resulted in lower FIB,FⅧ,FⅫ,and PC levels compared to white light LED （P<0.000??1,P<0.000??1,P=0.023??2,P<0.000??1） and fluorescent lamps （P<0.000??1,P<0.000??1,P=0.003??4,P<0.000??1）.Fluorescent lamps treatment resulted in higher FII levels than red light LED （P=0.012??2）,and higher FX levels than both white light LEDs and red light LEDs （P=0.036??6,0.006??8）.White light LEDs treatment yielded higher vWF:Ag levels than red light LED and fluorescent lamps （P=0.014??3,0.017??2）.Following MB/light treatment with the three light sources,the Sindbis virus titer in plasma decreasedby>4 LogTCID₅₀/0.1??mL.Conclusion All three MB/light treatments effectively inactivated Sindbis virus in plasma.Red light LED had the most significant effect on FIB and FⅧ activity.White light LED is the preferred light source for future plasma viral inactivation treatments.更多还原