【摘要】 目的 探讨慢性肾脏病（chronic kidney disease, CKD）患者外周血调节性T细胞（regulatory T cell, Treg）数量及其与血管钙化的相关性。方法 本研究为单中心横断面观察性研究，纳入2021年3月至2022年3月在复旦大学附属中山医院治疗的非透析CKD患者。采用腹部侧位X线评估腹主动脉钙化（abdominal aortic calcification, AAC）情况，流式细胞仪检测Treg数量和细胞因子水平。采用logistic回归分析CKD患者发生AAC的相关因素。结果 共纳入CKD患者83例，年龄17～86岁，男性57例（68.7%）。CKD分期：G1期7例（8.4%）,G2期17例（20.5%）,G3期21例（25.3%）,G4期19例（22.9%）,G5期19例（22.9%）。G1期和G2期患者未见AAC,G3～5期患者AAC发生率分别为23.8%、21.1%和26.3%。相较于G1期患者，G3～5期患者外周血Treg数量降低，白细胞介素（interleukin, IL）-6和IL-17F水平升高（P<0.05）。外周血Treg数量预测CKD患者发生AAC的受试者工作特征曲线下面积为0.766(95%CI 0.652～0.879, P=0.002)。Logistic回归分析显示，Treg数量降低是CKD患者发生AAC的相关因素（OR=0.957,95%CI 0.922～0.992,P=0.018）。结论 随着CKD进展，患者外周血Treg数量明显下降，且与CKD患者发生AAC相关。更多还原

【Abstract】 Objective To explore the number of peripheral blood regulatory T cells(Treg) in patients with chronic kidney disease(CKD) and its correlation with vascular calcification. Methods This was a single-center, cross-sectional, and observational study. Non-dialysis patients with CKD treated at Zhongshan Hospital, Fudan University from March 2021 to March 2022 were enrolled. Abdominal aortic calcification(AAC) was assessed using lateral abdominal X-ray. Number of Treg and cytokine levels were measured by flow cytometry. Logistic regression analysis was performed to evaluate the related factors for AAC in CKD patients. Results A total of 83 patients were included, aged 17–86 years, with 57 males(68.7%). The distribution of CKD stages was as follows: stage G1 in 7 patients(8.4%), stage G2 in 17 patients(20.5%), stage G3 in 21 patients(25.3%), stage G4 in 19 patients(22.9%), and stage G5 in 19 patients(22.9%). No AAC was observed in patients with stages G1 and G2, while the prevalence of AAC in patients with stages G3, G4, and G5 was 23.8%, 21.1%, and 26.3%, respectively. Compared with stage G1 patients, those with stages G3–5 showed decreased number of peripheral blood Treg and elevated levels of interleukin(IL)-6 and IL-17F(P<0.05). The area under the receiver operating characteristic curve for number of peripheral blood Treg in predicting AAC in CKD patients was 0.766(95%CI 0.652–0.879, P=0.002). Logistic regression analysis showed that decreased number of Treg was related factor for AAC in CKD patients(OR=0.957, 95%CI 0.922–0.992, P=0.018). Conclusion As CKD progresses, number of peripheral blood Treg significantly decreases, which is correlated with AAC in CKD patients.更多还原