【摘要】 对6年生福建柏(Chamaecyparis hodginsii)进行N肥300 g、P肥200 g、K肥50 g、Mg肥18.2 g施肥处理，采用水蒸气蒸馏法提取其叶精油。使用GC-MS对精油成分进行分析，以食品中常见的3种致病细菌(金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌)和3种致病真菌(黄曲霉、黑曲霉、桔青霉)为参试菌进行体外抑菌活性研究，探究施肥处理后福建柏叶精油成分及其抑菌活性。果表明:经2次施肥处理后，从福建柏叶精油中鉴定出52种有效成分，其中GC含量> 1%的主要成分有15种，总GC含量为94.10%,GC含量最高的化学成分有α-蒎烯(51.51%)和D-柠檬烯(9.19%)。福建柏叶精油对金黄色葡萄球菌和黄曲霉高度敏感，对大肠杆菌、枯草芽孢杆菌和桔青霉中度敏感，对黑曲霉低度敏感;精油对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌、黄曲霉、黑曲霉、桔青霉的最低抑菌体积分数(MIC)分别为5%、10%、2.5%、2.5%、10%、5%，最小杀菌体积分数(MBC)分别为10%、10%、5%、5%、10%、10%。福建柏叶精油对6种参试菌均具有明显抑制作用，表现出广谱抑菌性。更多还原

【Abstract】 This study investigated the effects of N 300 g,P 200 g,K 50 g,Mg 18.2 g fertilization treatments on the chemical composition and antimicrobial activity of the essential oil extracted from the leaves of 6-year-old Chamaecyparis hodginsii.The essential oil was obtained by hydrodistillation, and its chemical composition was analyzed using GC-MS.The in vitro antimicrobial activity was evaluated against 3 common foodborne pathogenic bacteria(Staphylococcus aureus,Escherichia coli,and Bacillus subtilis),and 3 pathogenic fungi(Aspergillus flavus,Aspergillus niger,and Penicillium citrinum).The results showed that 52 compounds were identified from the essential oil after 2 fertilization treatments.Among them,there were 15 main components with a GC content greater than 1%,and the total GC content was 94.10%,with α-pinene(51.51%) and D-limonene(9.19%) being the major constituents.The essential oil exhibited significant and broad-spectrum antimicrobial activity against all tested strains.It was highly sensitive to S.aureus and A.flavus,moderately sensitive to E.coli,B.subtilis,and P.citrinum,and less sensitive to A.niger.The minimum inhibitory volume fraction(MIC) values for S.aureus,E.coli,B.subtilis,A.flavus,A.niger,and P.citrinum were 5%,10%,2.5%,2.5%,10%,and 5%,respectively.The minimum bactericidal fungicidal volume fraction(MBC) values were 10%,10%,5%,5%,10%,and 10%,respectively.In conclusion,the essential oil from C.hodginsii leaves demonstrates notable broad-spectrum antimicrobial activity,which is influenced by fertilization treatment.更多还原