【摘要】 目的:探究表没食子儿茶素没食子酸酯壳聚糖纳米粒(epigallocatechingallate-chitosan nanoparticles, EGCG-NPs)掺入玻璃离子水门汀(glass ionomer cement, GIC)提升其物理性能和抗菌性能的最佳整合浓度。方法:采用离子交联法制备EGCG-NPs。以纳米粒形态、粒径分布、Zeta电位、多分散系数和释放研究来表征纳米粒；以材料的抗压强度、老化性能、吸水溶解性、氟离子释放、抗菌性能为指标，按照掺入不同质量分数(0.5、1、2、3、4、5 wt.%)的EGCG-NPs制备试样并进行筛选，并进行细胞毒性评价。结果:在28 d内，随着EGCG-NPs添加量的增加，抗压强度先增大后减小，在1 wt.%达到峰值；材料的吸水溶解率随EGCG-NPs添加量的增加影响增大；GIC抗菌性能和氟离子释放水平的强弱与添加EGCG-NPs的浓度呈正比；各组材料均无细胞毒性。结论:添加1 wt.%的EGCG-NPs能最大限度提高GIC的抗压强度和抗菌能力，显著降低GIC的溶解率，不影响吸水率，有提升氟离子释放的趋势。更多还原

【Abstract】 Objective: To explore the optimal integration concentration of epigallocatechin gallate-chitosan nanoparticles(EGCG-NPs) incorporated into glass ionomer cement(GIC) for enhancing its physical and antibacterial properties. Methods: EGCG-NPs were prepared via the ionic crosslinking. The nanoparticles were characterized by morphology, particle size distribution, zeta potential, polydispersity index(PDI), and release behavior. Specimens were prepared by adding EGCG-NPs at different mass fractions(0.5, 1, 2, 3, 4, 5 wt.%) and screened based on indicators including compressive strength, aging resistance, water absorption and solubility, fluoride ion release, and antibacterial activity. Cytotoxicity evaluation was also performed. Results: Within 28 days, the compressive strength of GIC first increased and then decreased with the increase of EGCG-NPs content, reaching a peak at 1 wt.%. The water absorption and solubility of the material were increasingly affected by the addition of EGCG-NPs. The antibacterial activity and fluoride ion release level of GIC were positively correlated with the concentration of EGCG-NPs. All groups of materials showed no cytotoxicity. Conclusion: The addition of 1 wt.% EGCG-NPs can maximize the compressive strength and antibacterial ability of GIC, significantly reduce its solubility(P<0.05), without affecting water absorption, and exhibit a trend of enhancing fluoride ion release.更多还原