【摘要】 目的:基于稀土元素掺杂的氟基磷灰石颗粒(FYE)制备一种利用金纳米颗粒(AuNPs)淬灭荧光并通过荧光强度指示葡萄糖浓度的上转换荧光传感器FYE@Au。方法:制备FYE@Au并进行微观形貌及结构观察；通过CCK-8法和细胞免疫荧光染色对FYE@Au的生物相容性进行检测；通过稳态荧光光谱仪检测FYE@Au的荧光强度和葡萄糖浓度的关系，以及FYE@Au对葡萄糖响应的可重复性。结果:FYE@Au表面成功修饰了5～10 nm的薄层二氧化硅，并有AuNPs覆盖；FYE@Au在0～100μg/mL浓度范围内具有良好的生物相容性，且FYE与FYE@Au均具有良好的血细胞相容性；稳态荧光光谱结果显示葡萄糖浓度在0～20 mmol/L的范围内和荧光强度呈现良好的线性关系，且FYE@Au多次重复测量的最大荧光强度基本不变，表明传感器对葡萄糖的响应具有可重复性。结论:FYE@Au在0～100μg/mL的浓度范围内具有良好的生物相容性，并且可以检测一定范围内的葡萄糖浓度，其检测能力具有可重复性。更多还原

【Abstract】 Objective: Based on rare earth element-doped upconversion fluorescent particles(FYE), an upconversion fluorescent sensor FYE@Au was fabricated, which utilizes gold nanoparticles(AuNPs) to quench fluorescence and indicates glucose concentration through fluorescence intensity. Methods: The microscopic morphology and structure of the fluorescent sensor FYE@Au were characterized using methods such as transmission electron microscopy. The biocompatibility of FYE@Au was evaluated using the CCK-8 assay and cellular immunofluorescence staining. The relationship between the fluorescence intensity of the sensor and the glucose concentration, as well as the repeatability of the sensor’s response to glucose, was measured by steady-state fluorescence spectrometer. Results: The surface of FYE@Au was successfully modified with a thin layer of silica at 5-10 nm and covered with AuNPs. FYE@Au exhibits good biocompatibility within the concentration range of 0-100 μg/mL, and both FYE and FYE@Au demonstrate good blood cell compatibility. Steady-state fluorescence spectroscopy results demonstrated a good linear relationship between glucose concentration(within the range of 0-20 mmol/L) and fluorescence intensity. Moreover, the maximum fluorescence intensity of FYE@Au remained largely unchanged after multiple repeated measurements, indicating that the sensor’s response to glucose is repeatable. Conclusions: FYE@Au exhibits good biocompatibility within the concentration range of 0-100 μg/mL and is capable of detecting glucose concentrations within a certain range, with reproducible detection capability.更多还原