【摘要】 目的 探讨血清涎液化糖链抗原-6(KL-6)、Wnt诱导分泌蛋白-1(WISP1)对肺炎支原体肺炎(MPP)患儿肺实变的早期诊断价值。方法 选择2023年2月至2024年5月在鄂州市中心医院就诊并确诊的90例MPP患儿作为研究组,并选择同期在鄂州市中心医院体检的同年龄段体检健康儿童96例作为对照组。根据患儿肺部影像学检查结果将研究组患儿分为实变组和非实变组。采用酶联免疫吸附试验(ELISA)检测所有研究对象血清KL-6、WISP1水平;采用多因素Logistic回归分析影响MPP患儿肺实变的相关因素;采用受试者工作特征(ROC)曲线评价血清KL-6、WISP1对肺实变的早期诊断效能,并使用De Long检验进行曲线下面积(AUC)比较。结果 研究组血清KL-6、WISP1水平高于对照组(P<0.05)。实变组血清KL-6、WISP1、C反应蛋白(CRP)水平及中性粒细胞百分比(N%)高于非实变组(P<0.05)。多因素Logistic回归分析结果显示,血清KL-6、WISP1水平升高均是MPP患儿发生肺实变的独立危险因素(P<0.05)。血清KL-6、WISP1单独及2项联合诊断MPP患儿肺实变的AUC分别为0.880、0.821、0.927,2项联合诊断的AUC大于KL-6、WISP1单独诊断的AUC(Z=1.961、2.599,P<0.05)。结论 MPP患儿及发生肺实变的患儿血清KL-6、WISP1水平均升高,2项联合诊断MPP患儿肺实变具有一定临床价值。更多还原

【Abstract】 Objective To investigate the early diagnostic value of serum krebs von den lungen 6(KL-6) and Wnt1 inducible signaling pathway protein 1(WISP1) expression levels for pulmonary consolidation in children patients with Mycoplasma pneumoniae pneumonia(MPP).Methods A total of 90 children patients with definitely diagnosed MPP in Ezhou Central Hospital from February 2023 to May 2024 were selected as the study group,and 96 healthy children at same age group who underwent the physical examinations in this hospital were selected as the control group.Based on the results of pulmonary imageologic examination,the study group was divided into the consolidation group and non-consolidation group.The enzyme linked immunosorbent assay(ELISA) was applied to detect the expression levels of serum KL-6 and WISP1.The multivariate Logistic regression was employed to screen the related factors affecting pulmonary consolidation in children patients with MPP.The receiver operating characteristic(ROC) curves were used to evaluate the early diagnostic performance of serum KL-6 and WISP1 for pulmonary consolidation,and the areas under the curve(AUC) conducted the comparison by using the De Long test.Results The serum KL-6 and WISP1 levels in the study group were higher than those in the control group(P<0.05).The serum KL-6,WISP1 and C-reactive protein(CRP) levels and neutrophil percentage(N%) in the consolidation group were significantly higher than those in the non-consolidation group(P <0.05).The multivariate Logistic regression results showed that the increase of serum KL-6 and WISP1 levels were the independent risk factors for lung consolidation occurrence in the children patients with MPP(P<0.05).The AUCs of serum KL-6 and WISP1 alone and their combination for the diagnosis of pulmonary consolidation in the children patients with MPP were 0.880,0.821 and 0.927 respectively,and the AUC of the 2-item combination diagnosis was greater than that of KL-6 and WISP1 alone(Z=1.961,2.599,P<0.05).Conclusion Serum KL-6 and WISP1 levels in the children patients with MPP and pulmonary consolidation occurrence are elevated and the 2-item combination for diagnosing pulmonary consolidation has certain clinical value.更多还原