【摘要】 目的：探索上调TNFAIP3对直肠癌皮下成瘤小鼠癌组织凋亡及caspase-3的影响。方法：TNFAIP3高表达慢病毒干预0、3、7、14??d测量肿瘤重量及体积，取材后应用ELISA检测法检测IL-6、IL-10炎症因子表达水平，应用流式检测法及免疫印记法检测凋亡率及caspase-3表达。结果：干预第7天与干预第14天检测肿瘤体积发现，与NS组比较，TNFAIP3高表达组肿瘤体积显著减少：第7天检测肿瘤体积：NS组：（297.9±??62.93）,TNFAIP3高表达组：（202.6±59.91）；第14天检测肿瘤体积：NS组：（481.1±79.67）,TNFAIP3高表达组：（293.2±103.25）。ELISA检测结果显示，与NS组比较，TNFAIP3高表达组IL-6与IL-10炎症因子的表达均显著降低：IL-6表达值：NS组：（32.55±11.87）,TNFAIP3高表达组：（21.02±10.97）;IL-10表达值：NS组：（163.62±47.17）,TNFAIP3高表达组：（93.26±39.03）。流式凋亡及免疫印迹结果显示，与NS组比较，TNFAIP3高表达组凋亡细胞比例及caspase-3活性均显著下调：免疫印迹结果caspase-3灰度表达值：NS组：（0.86±0.02）,TNFAIP3高表达组：（0.61±0.03）。结论：上调TNFAIP3可调控caspase-3途径促细胞凋亡，为直肠癌的治疗提供新靶点。更多还原

【Abstract】 Objective: This study explores the effect of upregulating TNFAIP3 on apoptosis and caspase-3 expression in subcutaneous tumorigenic mice with rectal cancer.Methods: Tumor weight and volume were measured at 0, 3, 7, and 14 days of treatment. After sampling, ELISA was used to detect the expression of IL-6 and IL-10 inflammatory factors, and flow cytometry and immunoblotting were used to detect apoptosis rate and caspase-3 expression.Results: On the 7 th and 14 th days of medication, tumor volume was significantly reduced in the TNFAIP3 overexpression group compared to the NS group. On the 7 th day, tumor volume was(297.9 ± 62.93) in the NS group and(202.6 ± 59.91) in the TNFAIP3 overexpression group. On the 14 th day, tumor volume was(481.1 ± 79.67) in the NS group and(293.2 ± 103.25) in the TNFAIP3 overexpression group; The ELISA test results showed that compared with the NS group, the expression of IL-6 and IL-10 inflammatory factors in the TNFAIP3 overexpression group was significantly reduced. The IL-6 expression value was(32.55 ± 11.87) in the NS group,(21.02 ± 10.97) in the TNFAIP3 overexpression group,(163.62 ± 47.17) in the NS group, and(93.26 ± 39.03) in the TNFAIP3 overexpression group. Flow cytometry and immunoblotting results showed that compared with the NS group, the proportion of apoptotic cells and caspase-3 activity in the TNFAIP3 overexpression group were also significantly reduced and down regulated. The grayscale expression value of caspase-3 in the immunoblotting results was(0.86 ± 0.02) in the NS group and(0.61 ± 0.03) in the TNFAIP3 overexpression group.Conclusion: Upregulation of TNFAIP3 can regulate the caspase-3 pathway and promote cell apoptosis, providing a new target for the treatment of rectal cancer.更多还原