【摘要】 为明确光环境处理对‘高山圣火’月季组培苗增殖生长和生理特性的影响，以无菌茎段为外植体，在组培增殖培养过程中设置6种不同光环境处理，分析光环境处理对组培苗增殖系数、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及玉米素核苷(ZR)含量的影响。结果表明，光环境处理对‘高山圣火’月季组培苗的增殖性状和生理指标均产生显著影响(P<0.05)。红光1蓝光1每日补光12 h处理下组培苗增殖系数最高，同时具有较高的SOD活性和ZR含量以及较低的MDA含量，说明该处理提高了‘高山圣火’月季组培苗对活性氧的解毒能力，减轻了细胞膜受氧化损伤程度，提高了内源激素ZR含量，是‘高山圣火’月季组培苗增殖的较佳光环境条件。更多还原

【Abstract】 In order to clarify the effects of light environment treatments on the growth and physiological characteristics of tissue culture Rosa hybrida ‘Alpine flame’ seedlings during the proliferation stage, six different light environment treatments were set up during the proliferation culture stage by using sterile stem segments as explants. The effects of these treatments on the proliferation coefficient, superoxide dismutase(SOD) activity, malondialdehyde(MDA) content and zeatin riboside(ZR) content of tissue culture seedlings were analyzed. The results showed that light environment treatments had significant effects on the proliferation traits and physiological indicators(P < 0.05). Under the treatment with the blue and red photon flux ratio of 1∶1 for supplementing light 12 h daily, the proliferation coefficient was highest, the SOD activity and ZR content were higher, and the MDA content was lower. That indicated that the treatment improved the detoxification ability of tissue culture seedlings to reactive oxygen, reduced the degree of oxidative damage to cell membranes, and increased the endogenous hormone ZR content. The treatment with the blue and red photon flux ratio of 1∶1 for supplementing light 12 h daily was the optimal light environment condition for the proliferation of tissue culture Rosa hybrida ‘Alpine flame’ seedlings.更多还原