【摘要】 为探究肉羊同期发情超数排卵及胚胎移植效果，研究采用3种不同梯度促卵泡素(FSH)激素方案对45只白头杜泊母羊进行同期发情和超数排卵处理，筛选最优方案，对白头杜泊羊、黑头杜泊羊和多胎萨福克羊各20只进行超数排卵，探究黄体存在或缺失对不同品种绵羊超数排卵反应和胚胎产量的影响，比较自然交配、宫颈输精和腹腔镜输精的受精率，并进行鲜胚和冻胚移植对比分析，旨在优化输精技术与胚胎移植技术体系。结果表明，3种方案的可用胚胎数均在88%以上，方案2只均冲胚数显著高于方案3(P<0.05),但只均可用胚胎数3组间无显著差异(P>0.05);白头杜泊羊、多胎萨福克羊的黄体数显著高于黑头杜泊羊黄体数(P<0.05),而卵巢黄体数量与胚胎存活率无显著相关性(P>0.05);腹腔镜输精技术受胎率优于自然交配、宫颈口人工授精受胎率(P<0.05);鲜胚组胚胎移植受胎率(67.93%)高于冻胚组(55.65%)(P<0.05)。综上所述，在本实验条件下，采用方案2(FSH总剂量400 IU,递减注射40～70 IU/次)对肉羊进行超排处理后，结合腹腔镜鲜胚移植技术，可显著提升胚胎移植效率。更多还原

【Abstract】 To explore the effect of synchronized estrus, superovulation and embryo transfer in meat sheep, this study compared three different follicle-stimulating hormone(FSH) schemes to induce synchronized estrus and superovulation in 45 white Dorper ewes, and screened the optimal scheme. The superior scheme was then used to superovulate white Dorper, black Dorper and multi-twin Suffolk sheep to investigate the effect of luteal presence or absence on superovulation response and embryo yield in different sheep breeds. Meanwhile, the fertilization rates of natural mating, cervical insemination and laparoscopic insemination were compared, and the fresh and frozen embryos collected from superovulated donors were compared in terms of embryo transfer. The aim was to optimize the insemination and embryo transfer technology system. The results showed that the number of transferable embryos in all three schemes was above 88%. The average number of embryos recovered per ewe in Scheme 2 was significantly higher than that in Scheme 3(P<0.05), but there was no significant difference in the average number of transferable embryos among the three groups(P>0.05). The number of corpora lutea in white Dorper and multi-twin Suffolk sheep was significantly higher than that in black Dorper sheep(P<0.05), but there was no significant correlation between the number of ovarian corpora lutea and embryo survival rate(P>0.05). The conception rate of laparoscopic insemination was significantly higher than that of natural mating and cervical artificial insemination(P<0.05). The conception rate of fresh embryo transfer(67.93%) was higher than that of frozen embryo transfer(55.65%)(P<0.05). In conclusion, under the experimental conditions of this study, the use of Scheme 2(total FSH dose of 400 IU, decreasing injection of 40～70 IU per time) for superovulation in meat sheep, combined with laparoscopic fresh embryo transfer technology, can significantly improve the efficiency of embryo transfer.更多还原