【摘要】 为了探究人工湿地对海水养虾尾水的处理效果及植物根系与不同基质之间微生物群落的分布规律，本文构建了复合垂直潜流人工湿地，对海水养虾尾水污染物的去除效果进行研究，并通过高通量测序技术分析了人工湿地微生物群落结构特征。结果表明，COD、NH⁺₄-N、NO^-₂-N、NO^-₃-N的去除率分别为41.370%±6.370%、70.330%±6.040%、73.810%±7.140%、67.260%±7.570%,该系统对有机物和营养盐去除效果较好，且下行池的去除效果优于上行池。植物根系和各基质层微生物群落的组成相似；代谢功能丰富；但多样性存在明显差异，相较于植物和其他基质层，珊瑚石层微生物群落多样性最低，本系统植物根系和各基质层主要的优势菌门为变形菌门（Proteobacteria）、拟杆菌门（Bacteroidota）、厚壁菌门（Firmicutes）、放线菌门（Actinobacterota）、脱硫杆菌门（Desulfurobacteria）;优势菌纲为：α-变形菌纲（Alphaproteobacteria）、γ-变形菌纲（Gammaproteobacteria）、拟杆菌纲（Bacteroidia）、芽孢杆菌纲（Bacilli）、放线菌纲（Actinobacteria）;丰度较高的菌属有芽孢杆菌属（Bacillus）、rank-f-unclassified、unclassified-f-Flavobacteriaceae、Aquibacter、unclassified-f-Rhodobacteraceae、硝化螺菌属（Nitrospira）、硝酸盐还原菌属（Nitratireductor）、Methylotenera、噬甲基菌（Methylophaga）、拉布伦茨菌属（Labrenzia）。硝化螺菌属（Nitrospira）主要分布在植物根系和细砂层，而反硝化菌属（如芽孢杆菌属）主要分布在溶氧较低的煤渣层和珊瑚石层。更多还原

【Abstract】 In order to explore the performance of constructed wetlands in treating seawater shrimp tail water, and the distribution pattern of microbial communities among and between plant roots and different substrates, in this study, we constructed a composite vertical subsurface flow constructed wetland to study the removal effect of pollutants in seawater shrimp tail water, and characterized the microbial community structure of constructed wetlands using high-throughput sequencing technology. The results showed that the system has a high removal effect on nutrients. The removal rate of COD, NH⁺₄-N, NO^-₂-N and NO^-₃-N of the shrimp tail water by the CW selected in this study reached 41.370 ± 6.370%, 70.330 ± 6.040%, 73.810 ± 7.140% and 67.260 ± 7.570%, respectively, and the removal effect of the downstream pool was better than upstream pool. The composition of microbial communities in plant roots and substrates was similar, but there were significant differences in diversity. Compared with plants and other substrates, the diversity of microbial communities in coral layer was the lowest. Proteobacteria, bacteroidota, Firmicutes, actinobacterota and desulfurobacteria were the dominant phylum. α-Alphaproteobacteria, γ-Gammaproteobacteria, bacteroidia, bacillus, actinobacteria were the dominant class. The bacteria with high abundances were Bacillus, rank-f-unclassifie, unclassified-f-Flavobacteriaceae, Aquibacter, unclassified-f-rhodobacteraceae, Nitrospira, Nitratireductor, Methylotenera, Methylophaga, Labrenzia. Nitrospira which mainly distributed in plant roots and fine sand layer, while denitrifying bacteria（such as Bacillus） mainly distributed in coal cinder layer and coral layer with low dissolved oxygen.更多还原