【摘要】 目的:探索马归液含药血清对膀胱癌(bladder cancer,BC)细胞的增殖、凋亡与迁移能力的影响，以及调控胆固醇合成的机制。方法:T24细胞分为对照组、空白血清组及马归液含药血清(100%、50%、10%)组，通过CCK-8法检测细胞增殖活力，流式细胞术检测细胞凋亡，Transwell实验检测细胞迁移能力，Western blot法检测SREBP、HMGCR、SQLE蛋白表达水平。结果:与对照组比较，马归液含药血清组T24细胞的增殖活力、迁移能力以及SREBP、HMGCR、SQLE蛋白表达水平均显著降低，细胞凋亡率明显升高(P<0.001)。结论:马归液可能通过调控胆固醇合成发挥抑制BC细胞增殖与迁移的作用。更多还原

【Abstract】 Objective: To explore the effects of Ma Gui Liquid-containing serum on the proliferation,apoptosis,and migration ability of bladder cancer(BC) cells,and the mechanism of regulating cholesterol synthesis. Methods: T24 cells were divided into control group,blank serum group,and Ma Gui Liquid-containing serum groups(100%,50%,10%). Cell proliferation activity was detected by CCK-8 assay,cell apoptosis was detected by flow cytometry,cell migration ability was detected by Transwell assay,and the protein expression levels of SREBP,HMGCR,and SQLE were detected by Western blot. Results: Compared with the control group,the proliferation activity,migration ability,and protein expression levels of SREBP,HMGCR,and SQLE in T24 cells of the Ma Gui Liquid-containing serum group were significantly reduced,and the cell apoptosis rate was significantly increased(P < 0. 001). Conclusion: Ma Gui Liquid may inhibit bladder cancer cell proliferation and migration by regulating cholesterol synthesis.更多还原