【摘要】 为探究不同稀释比例对鸭精液人工授精效果的影响，研究以临武鸭为试验对象，使用EK2稀释液按1∶1和1∶3比例稀释后进行输精，测定稀释后的精子活力、精子活率及运动参数（直线速度、曲线速度、平均路径速度），并计算种蛋受精率及受精蛋孵化率。结果表明：稀释后4 h,EK2稀释液处理的精子活力、精子活率及运动参数均极显著高于生理盐水处理；稀释后6 h,EK2稀释液1∶3组的精子活力极显著高于1∶1组；输精后1～6 d,EK2稀释液1∶1组、1∶3组和原精组的受精率及受精蛋孵化率均无显著差异；输精后7～12 d，原精组的受精率极显著高于EK2稀释液1∶1组和1∶3组。日受精率趋势显示，输精后1～4 d,EK2稀释液1∶1组与1∶3组的日受精率高于原精组；输精后5～12 d,2个稀释组的日受精率均低于原精组。试验表明，使用EK2稀释液按1∶3或1∶1比例稀释后输精（有效精子数不低于1.0×10~8个），可获得较为理想的受精率，结合实际受精率变化趋势，建议在生产中每间隔4 d输精1次。更多还原

【Abstract】 This study aimed to explore the effect of different dilution ratios of duck semen on artificial insemination. Semen was collected from Linwu ducks and diluted at ratios of 1 ∶ 1 and 1 ∶ 3 with EK2 diluent for artificial insemination. Post-dilution sperm motility, sperm viability, and sperm motility parameters [straight-line velocity(VSL), curvilinear velocity(VCL), and average path velocity(VAP)], fertility rate, and hatchability were assessed. The results showed that at the time point of 4 h post-dilution, the sperm motility, viability, and motility performance of both 1 ∶ 1 and 1 ∶ 3 dilution groups were extremely significantly higher than that in the normal saline dilution group. At the time point of 6 h post-dilution, sperm motility in the 1 ∶ 3 dilution group was extremely significantly higher than that in the 1 ∶ 1 dilution group. During 1–6 d post-insemination, the average fertility rates and hatchability of the 1 ∶ 1 dilution, 1 ∶ 3 dilution, and original semen groups had no significant differences. During 7–12 d, the average fertility rate of the original semen group was extremely significantly higher than those of the 1 ∶ 1 and 1 ∶ 3 dilution groups. Daily fertility rate analysis revealed that the 1 ∶ 1 and 1 ∶ 3 dilution groups had higher fertility rate than the original semen group during 1–4 d and had lower fertility rate during 5–12 d. In conclusion, ideal fertility can be achieved by inseminating semen diluted with EK2 diluent at a ratio of 1∶3 or 1 ∶ 1(with an effective sperm count of no less than 1.0×10~8). Considering the fertility trend, it was recommended to perform artificial insemination every 4 d in production.更多还原