【摘要】 为了探究连城白鸭不同组织的黑色素沉积规律，试验以连城白鸭为研究对象，通过苏木精-伊红(H.E.)和甲苯胺蓝(TB)染色法对各组织(喙皮、蹼部皮肤、胸部皮肤和肝脏)进行形态学观察，测定喙皮、蹼部皮肤、胸部皮肤、胸部肌肉和肝脏的黑色素含量，并利用实时荧光定量PCR(RT-qPCR)测定各组织中黑色素生成相关基因(PI3K、AKT、ILK、GSK3β、MC1R、TYR、TYRP1、TYRP2)的相对表达量。结果表明：喙皮、蹼部皮肤和胸部皮肤组织均可观察到黑色素颗粒，且黑色素颗粒主要分布在表皮基底层及毛囊周围；黑色素含量为喙皮>肝脏>蹼部皮肤>胸部肌肉>胸部皮肤；喙皮和蹼部皮肤PI3K基因相对表达量显著低于胸部皮肤和胸部肌肉(P<0.05),喙皮PI3K基因相对表达量显著低于肝脏(P<0.05),喙皮和蹼部皮肤AKT基因相对表达量显著低于其他组织(P<0.05),喙皮和蹼部皮肤ILK、GSK3β、MC1R、TYR、TYRP1和TYRP2基因相对表达量显著高于其他组织(P<0.05)。说明PI3K、AKT和ILK等黑色素生成相关基因的表达可能会影响黑色素的沉积。更多还原

【Abstract】 In order to explore the pattern of melanin deposition in different tissues of Liancheng white ducks, Liancheng white ducks were used as the research objects. The morphology of various tissues(beak skin, web skin, chest skin, and liver) was observed by hematoxylin-eosin(H.E.) and toluidine blue(TB) staining, and the melanin content in the beak skin, web skin, chest skin, chest muscle and liver was determined. Additionally, the expression levels of melanin-related genes(PI3K, AKT, ILK, GSK3β, MC1R, TYR, TYRP1, TYRP2) in each tissue were quantified by real-time fluorescence quantitative PCR(RT-qPCR). The results showed that melanin granules were observed in the beak skin, web skin, and chest skin tissues, and were primarily distributed in the basal layer of the epidermis and around the hair follicles. The melanin content was ranked as follows: beak skin>liver>web skin>chest muscle>chest skin. The relative expression of the PI3K gene in beak skin and web skin was significantly lower than that in chest skin and chest muscle(P<0.05). The relative expression of the PI3K gene in beak skin was significantly lower than that in liver(P<0.05). The relative expression of the AKT gene in beak skin and web skin was significantly lower than that in other tissues(P<0.05). The relative expression of ILK, GSK3β, MC1R, TYR, TYRP1, and TYRP2 genes in beak skin and web skin was significantly higher than that in other tissues(P<0.05). These results indicated that the expression of melanin-related genes such as PI3K, AKT, and ILK might influence melanin deposition.更多还原