【摘要】 目的 检测长链非编码RNA (long non-codingRNA,lncRNA) MELTF-AS1在膀胱癌组织及细胞中的表达，分析其表达与膀胱癌患者肿瘤分期(T分期)之间的关系，并探究MELTF-AS1对细胞增殖、迁移及侵袭等生物学行为的影响。方法 收集2021年9月至2022年10月在河北医科大学第四医院行手术治疗并经病理确诊的42例膀胱癌患者癌组织及其相对应的正常癌旁膀胱尿路上皮组织进行研究。采用实时荧光定量PCR(real time-qPCR)检测MELTF-AS1在膀胱癌组织及膀胱癌细胞株5637、T24、SW780和正常膀胱上皮细胞株SV-HUC-1中的表达水平，分析其与各临床参数之间的关系。构建过表达载体pcDNA3.1-MELTF-AS1与敲低载体si-MELTF-AS1,并分别转染5637、T24及SW780细胞。采用细胞增殖实验(MTS法)、克隆形成实验、划痕实验及Transwell小室侵袭实验，检测过表达或敲低MELTF-AS1对膀胱癌细胞增殖、迁移及侵袭的影响。结果 MELTF-AS1在膀胱癌组织中的相对表达量明显高于其相对应的正常癌旁膀胱尿路上皮组织，且与肿瘤浸润深度(T分期)相关，与非肌层浸润性膀胱癌患者相比，肌层浸润性膀胱癌患者的肿瘤组织中MELTF-AS1的表达水平更高，差异有统计学意义(P<0.05)。与SV-HUC-1细胞比较，MELTF-AS1的相对表达量在3株膀胱癌细胞系中均上调，且在5637细胞中MELTF-AS1的表达水平最高，差异有统计学意义(P<0.05)。敲低MELTF-AS1可显著抑制5637细胞的增殖、迁移和侵袭能力，而过表达MELTF-AS1可促进T24细胞的增殖、迁移和侵袭。结论 MELTF-AS1在膀胱癌组织及膀胱癌细胞中高表达，促进膀胱癌细胞增殖、迁移及侵袭等生物学行为。更多还原

【Abstract】 Objective To detect the expression of long non-coding RNA(lncRNA) MELTF-AS1 in bladder cancer(BC) tissues and cells, the relationship between its expression and tumor stage(T stage) of BC patients, and its impact on biological behaviors of BC(e.g., cell proliferation, migration and invasion).Methods Cancer tissues and corresponding normal bladder urinary tract epithelial tissue adjacent to cancer tissues were surgically collected from 42 BC patients who underwent surgical treatment and confirmed by pathology in the Fourth Hospital of Hebei Medical University from September 2021 to October 2022. The expression of MELTF-AS1 in BC tissues, BC cell lines(5637, T24, SW780) and normal bladder epithelial cell line(SV-HUC-1) was detected by real-time quantitative PCR. Additionally, the relationship between MELTF-AS1 level and clinical parameters in BC patients was analyzed. The overexpression vector pcDNA3.1-MELTF-AS1 and knockdown vector si-MELTF-AS1 were constructed and transfected into 5637, T24, and SW780 cells. The effects of overexpression or knockdown of MELTF-AS1 on the proliferation, migration and invasion of BC cells were detected by MTS assay, colony formation assay, wound healing assay, and Transwell invasion assay.Results The relative expression of MELTF-AS1 in BC tissue was significantly higher than that in its corresponding normal bladder urothelium adjacent to cancer tissue. Compared with non-muscle invasive BC patients, the expression level of MELTF-AS1 in muscle invasive tissues of BC patients was significant higher. Its expression was significantly related to the depth of tumor invasion(T stage). MELTF-AS1 was up-regulated in all three bladder cancer cell lines compared with SV-HUC-1 cells, with the highest expression in 5637 cells. Knockdown of MELTF-AS1 significantly inhibited the proliferation, migration, and invasion abilities of 5637 cells, while overexpression of it significantly promoted the proliferation, migration, and invasion abilities of T24 cells(P<0.05).Conclusion MELTF-AS1 is overexpressed in BC tissues and cells, which can promote the biological behaviors of proliferation, migration and invasion in BC cells.更多还原