【摘要】 目的 采用MACS免疫磁珠法分选乳腺癌细胞中CD44⁺CD24^-/low细胞亚群，探究丹参酮ⅡA对该细胞亚群的诱导分化作用。方法 运用MACS免疫磁珠法分选CD44⁺CD24^-/low细胞亚群，通过流式细胞术测定分选前后该细胞亚群的比例；将不同浓度的丹参酮ⅡA与CD44⁺CD24^-/low细胞亚群共培养，采用流式细胞术检测细胞周期状态，明确其在乳腺癌干细胞分化中的作用。结果 经MACS免疫磁珠法分选，成功获得约6%的CD44⁺CD24^-/low细胞，流式细胞术鉴定显示该细胞亚群比例达93.85%。不同浓度丹参酮ⅡA干预后，CD44⁺CD24^-/low细胞在G0/G1期、S期及G2/M期的分布比例呈现显著差异。随着丹参酮ⅡA浓度从0μg/mL升高至10μg/mL，细胞G0/G1期比例从（92.83±0.25）%持续下降至（84.07±0.15）%，表明丹参酮ⅡA对细胞周期进程具有显著的浓度依赖性调控作用。结论 MACS免疫磁珠法可成功分选获得CD44⁺CD24^-/low细胞亚群；丹参酮ⅡA能够促使更多该细胞亚群从静止的G0/G1期退出，进入活跃的增殖周期，加速DNA复制（S期）与细胞分裂（G2/M期）进程，进而增强细胞增殖活性。更多还原

【Abstract】 Objective To isolate the CD44⁺CD24^-/lowsubpopuLation from breast cancer cells using MACS immunomagnetic bead sorting,and to investigate the differentiation-inducing effect of tanshinoneⅡA on this cell subpopuLation.Methods The CD44⁺CD24^-/lowcell subpopuLation was sorted by MACS immunomagnetic bead sorting,and the proportion of this subpopuLation before and after sorting was determined by flow cytometry.After co-cuLturing the CD44⁺CD24^-/lowcell subpopuLation with different concentrations of tanshinone Ⅱ A,the cell cycle distribution was detected by flow cytometry to clarify the role of tanshinone Ⅱ A in the differentiation of breast cancer stem cells.Results Approximately 6% of CD44⁺CD24^-/lowcells were successfuLly obtained via MACS immunomagnetic bead sorting,and flow cytometric identification showed that the purity of this cell subpopuLation reached 93.85%.After intervention with different concentrations of tanshinone Ⅱ A,the distribution ratios of CD44⁺CD24^-/lowcells in G0/G1 phase,S phase and G2/M phase exhibited significant differences.As the concentration of tanshinone ⅡA increased from 0 μg/mL to 10 μg/mL,the proportion of cells in G0/G1 phase decreased continuously from 92.83±0.25% to 84.07±0.15%,indicating that tanshinoneⅡA exerted a remarkable concentration-dependent reguLatory effect on cell cycle progression.Conclusion The CD44⁺CD24^-/lowcell subpopuLation can be successfuLly isolated using MACS immunomagnetic bead sorting.Tanshinone Ⅱ A can promote more cells of this subpopuLation to exit the quiescent G0/G1 phase and enter the active proliferation cycle,accelerate the processes of DNA replication（S phase）and cell division（G2/M phase）,thereby enhancing cell proliferative activity.更多还原