【摘要】 为揭示地稍瓜染色体核型及基因组大小，为全基因组测序提供借鉴。采用荧光原位杂交对地稍瓜染色体进行核型分析，并以番茄（Solanum lycopersicum L.）基因组作为内参，采用流式细胞术对地稍瓜基因组大小进行评估，并结合高通量测序技术对地稍瓜进行基因组Survey分析，对其基因组大小、重复序列、杂合度及GC含量等特征信息进行评估。结果表明，地稍瓜染色体数为2n=2x=24，长0.5～1.5μm，为二倍体。估算其基因组大小约为163.60～215.04 Mb，重复序列比为44.05%，杂合度约1.68%,GC含量为34.16%，表明地稍瓜基因组属于高杂合的复杂基因组。更多还原

【Abstract】 To reveal the chromosome karyotype and genome size of Cynanchum thesioides and provide a reference for whole-genome sequencing, fluorescence in situ hybridization(FISH) was used for karyotype analysis of Cynanchum thesioides chromosomes. Using the genome of tomato(Solanum lycopersicum L.) as an internal reference, flow cytometry was employed to estimate the genome size of Cynanchum thesioides. Combined with high-throughput sequencing technology, a genome survey analysis was conducted to evaluate key genome characteristics, including genome size, repetitive sequence ratio, heterozygosity rate, and GC content. The results showed that the chromosome number of Cynanchum thesioides is 2n=2x=24, with chromosome lengths ranging from 0.5 to 1.5 μm, indicating it is a diploid species. The estimated genome size is approximately 163.60～215.04 Mb, with a repetitive sequence ratio of 44.05%, a heterozygosity rate of approximately 1.68%, and a GC content of 34.16%. These findings indicate that the genome of Cynanchum thesioides is a complex genome with high heterozygosity.更多还原