【摘要】 基于生物信息学分析裂褶菌活性成分抗枯草芽孢杆菌的潜在作用机制。利用PubMed、中国知网等数据库查阅并筛选出裂褶菌的主要活性成分，利用分子对接技术、KEGG通路、GO功能分析等方法探究裂褶菌活性成分抗枯草芽孢杆菌的潜在作用机制。结果表明，裂褶菌抗枯草芽孢杆菌的主要活性成分为Schizophyllan、Schizines B、Schizines A，主要通过DNA模板转录调控、细菌孢子形成激活调控、DNA-蛋白质复合物、转录顺式调控区结合、相同的蛋白质结合、DNA结合及DNA结合转录抑制因子活性和GO功能活性以及组氨酸激酶与其下游的反应调节子共同组成典型的双组分调节系统发挥抗枯草芽孢杆菌的作用。研究表明，裂褶菌可能通过多条通路、多种代谢途径调控多种逆境响应相关的基因表达，影响着细菌的孢子形成过程及细菌的总体代谢、细胞增殖及分裂等达到抗枯草芽孢杆菌的效果。更多还原

【Abstract】 To analyze the potential mechanism of action of the active components of Schizophyllum commune Fr. against Bacillus subtilis based on bioinformatics. The main active components of Schizophyllum commune Fr. were identified and screened by PubMed and China Knowledge Network, and the potential mechanism of action of the active components against Bacillus subtilis was investigated by molecular docking technology, KEGG pathway and GO function analysis. The results showed that the main active components of P. schizophylla against B. subtilis were Schizophyllum commune Fr, Schiz B and Schizines A, which had an antiBacillus subtilis effect through a typical two-component regulatory system consisting of DNA template transcriptional regulation, bacterial sp formation activation regulation, DNA-protein complex, transcriptional cis-regulatory element binding, the same protein binding, DNA binding and DNA binding transcriptional inhibitory factor activity function activity and histidine kinase and its downstream response regulator. To sum up, Schizophyllum commune Fr. may regulate the expression of a variety of genes related to multiple responses through multiple pathways and various metabolic pathways, affecting the process of bacterial spore formation and the overall metabolism, cell proliferation, and division of bacteria to achieve the effect of anti-Bacillus subtilis.更多还原