【摘要】 为调研现阶段流浪犬血液中的DNA病毒群落结构组成及其功能预测，该研究利用宏病毒组学技术对采集自深圳救助站的流浪犬血液进行宏病毒高通量测序，过滤并去除宿主序列，用小片段序列Reads分析结合组装序列Contigs分析，探究DNA病毒群落组成的特点。结果表明，流浪犬血液中DNA病毒群落的优势病毒目为疱疹病毒目（Herpesvirales）、有尾噬菌体目（Caudovirales）和环状病毒目（Cirlivirales），优势病毒属为水痘疱疹病毒属（Varicellovirus）和圆环病毒属（Circovirus）。此外，利用KEGG数据库和COG数据库对功能基因进行分析。结果表明，KEGG数据库分析得到7个一级分类功能，COG数据库分布在20个COG类别。功能类别主要集中在在可移动基因组、一般功能预测、遗传信息过程、功能层次结构、代谢和转录。揭示深圳地区流浪犬血液中DNA病毒群落中存在丰富的疱疹病毒、圆环病毒和噬菌体，提示现阶段深圳地区流浪犬可能存在规模性的免疫低下和潜在共感染，具有较重要的间接公共卫生意义，为进一步挖掘验证流浪犬血液DNA病毒潜在危害性和功能基因作用提供参考。更多还原

【Abstract】 To investigate the composition and functional potential of DNA viral communities in stray dog blood,this study employed viral metaviromics to perform high-throughput sequencing of blood samples collected from stray dogs at a rescue center in Shenzhen. Host-derived sequences were filtered and removed,followed by analysis of shortsequence reads and assembled contigs to characterize the DNA viral community structure. The results revealed that the dominant viral orders were Herpesvirales,Caudovirales,and Cirlivirales,while the predominant genera were Varicellovirus and Circovirus. Functional gene annotation was conducted using the KEGG and COG databases. KEGG analysis identified seven major functional categories,and COG classification covered 20 functional groups. These functional categories primarily included mobile genetic elements,general function prediction,genetic information processing,multi-level functional structures,metabolism,and transcription. This study demonstrating that the DNA virome in stray dog blood from the Shenzhen region is dominated by herpesviruses,circoviruses,and bacteriophages,suggesting potential widespread immunosuppression and co-infection risk within the local stray dog population. These findings highlight significant indirect public health implications and provide a foundation for further exploration of the pathogenicity and functional roles of DNA viruses in stray dog blood.更多还原