【摘要】 通过建立中波紫外线(UVB)诱导的小鼠皮肤光损伤模型，系统评估了芍药籽油乳液(PSOE)的光损伤修复作用.采用Franz扩散池法比较了PSOE与纯芍药籽油(PSO)的皮肤渗透与滞留性能；通过测定细胞内活性氧(ROS)水平评价了其抗氧化能力；建立动物模型以观察皮肤愈合情况、红斑程度及弹性变化，并开展了组织病理学分析(HE与Masson染色)及分子检测(ELISA与免疫组化法检测TNF-α, IL-1β, IL-6和MMP-1表达).实验结果表明，PSOE的皮肤累积渗透量比PSO提升约1.5倍，皮肤滞留率提高约4倍；其清除UVB诱导ROS的能力显著优于市售乳液(CE)及空白乳液基质(EB).动物实验结果表明，PSOE处理组创面愈合率在第7 d达(92.31±3.53)%，真皮层炎症细胞浸润减少，表皮厚度恢复近正常水平，胶原纤维密度增加.分子检测结果表明，PSOE使TNF-α, IL-1β, IL-6及MMP-1的表达下调.综上所述，PSOE可通过增强透皮递送、抑制氧化应激、减轻炎症反应、促进胶原再生及调节基质代谢等多途径协同作用，实现对UVB诱导皮肤光损伤的有效修复.更多还原

【Abstract】 By establishing a UVB-induced mouse skin photodamage model, the photodamage repairing effect of peony seed oil emulsion(PSOE) was systematically evaluated. In this work, the Franz diffusion cell method was used to compare the skin penetration and retention properties of PSOE and pure peony seed oil(PSO). The antioxidant capacity was evaluated by measuring the intracellular reactive oxygen species(ROS) level. An animal model was established to observe skin healing, erythema degree, and elasticity changes, and histopathological analysis(HE and Masson staining) and molecular detection(ELISA and immunohistochemistry to detect the expression of TNF-α, IL-1β, IL-6, and MMP-1) were carried out. The experimental results showed that the cumulative skin penetration amount of PSOE was approximately 1.5 times higher than that of PSO, and the skin retention rate increased by about 4 times. Its ability to scavenge UVB-induced ROS was significantly better than that of the commercial emulsion(CE) and the blank emulsion base(EB). Animal experiments indicated that the wound healing rate of the PSOE treated group reached(92.31±3.53)% on the 7 th day. The infiltration of inflammatory cells in the dermis decreased, the epidermal thickness recovered to nearly the normal level, and the density of collagen fibers increased. Molecular detection results showed that PSOE down-regulated the expression of TNF-α, IL-1β, IL-6, and MMP-1. In conclusion, PSOE effectively repairs UVB-induced skin photodamage through multiple synergistic pathways, including enhancing transdermal delivery, inhibiting oxidative stress, reducing the inflammatory response, promoting collagen regeneration, and regulating matrix metabolism.更多还原