【摘要】 合成、表征了2类（母体及铁、钴、镍3种过渡金属取代的磷钼酸）共4种Dawson型磷钼酸化合物H₆[P₂Mo₁₈O₆₂], H₈[P₂Mo₁₇Fe（OH₂）O₆₁], H₈[P₂Mo₁₇Co（OH₂）O₆₁]和H₈[P₂Mo₁₇Ni（OH₂）O₆₁](以下简称为P₂Mo₁₈,P₂Mo₁₇Fe, P₂Mo₁₇Co和P₂Mo₁₇Ni)，进一步将Dawson型磷钼酸化合物与VC（VC）复合，探究了复合物对α-葡萄糖苷酶的抑制作用.在此基础上，通过Dawson型磷钼酸与VC以不同比例复合，进行了相应的酶动力学实验，考察了4种不同比例的Dawson型磷钼酸与VC复合物对α-葡萄糖苷酶的抑制效果，并选取抑制效果最优的比例进行相应的抑制机理和抑制类型研究.结果表明，4种不同的Dawson型磷钼酸-VC复合物均对α-葡萄糖苷酶具有良好的抑制作用.当P₂Mo₁₈-VC复合比例为5∶1, P₂Mo₁₇Fe-VC和P₂Mo₁₇Ni-VC复合比例为3∶1, P₂Mo₁₇CoVC复合比例为2∶1时，对α-葡萄糖苷酶的抑制效果最好，4种Dawson型磷钼酸-VC复合物[P₂Mo₁₈, P₂Mo₁₇MVC(M=Ni²⁺, Co²⁺, Fe²⁺)]均表现为可逆的混合型抑制.研究结果表明，4种多酸与VC复合物具有开发为α-葡萄糖苷酶抑制剂的潜力.更多还原

【Abstract】 In this work, the inhibitory effects of Dawson-type polyoxometalates（POMs） complexed with vitamin C（VC） on α-glucosidase activity was investigated. Four Dawson-type phosphomolybdates were synthesized and characterized, including the parent compound H₆[P₂Mo₁₈O₆₂](P₂Mo₁₈) and three transition metal-substituted derivatives{H₈[P₂Mo₁₇Fe（OH₂）O₆₁](P₂Mo₁₇Fe), H₈[P₂Mo₁₇Co（OH₂）O₆(P₂Mo₁₇Co) and H₈[P₂Mo₁₇Ni（OH₂）O₆1(P₂Mo₁₇Ni)}. Enzyme kinetic studies revealed that all four POM-VC complexes exhibited significant inhibitory activity against α-glucosidase. The optimal inhibition ratios were determined to be 5∶1 for P₂Mo₁₈-VC, 3∶1 for P₂Mo₁₇Fe-VC and P₂Mo₁₇Ni-VC, and 2∶1 for P₂Mo₁₇Co-VC, with corresponding IC₅₀ values of 0.194, 2.507, 2.809, and 5.332 mmol/L, respectively. Mechanistic studies demonstrated that these complexes functioned as reversible mixed-type inhibitors. These findings suggest the potential of Dawson-type POM-VC complexes as novel α-glucosidase inhibitors for diabetes management.更多还原