【摘要】 为探讨盐（NaCl）胁迫对萱草‘大同黄花’幼苗生长及生理的影响，采用水培法，测定不同NaCl浓度（0, 100, 200, 400 mmol/L）和胁迫时间（0, 1, 3, 5, 7 d）对‘大同黄花’生长、生物量、内源激素、抗氧化酶活性、渗透物质含量。结果发现：NaCl浓度升高后，幼苗茎叶鲜质量、干质量和叶长、叶宽均逐渐下降；根系鲜质量和干质量呈先升后降的变化。随NaCl浓度的升高和胁迫时间的增加，各处理‘大同黄花’超氧阴离子自由基（O₂^-·）、过氧化氢（H₂O₂）、丙二醛（MDA）含量明显升高；NaCl浓度升高后，幼苗过氧化物酶（POD）、超氧化物歧化酶（SOD）活性表现为先升后降的变化，胁迫时间增加后，POD和SOD活性持续升高。而过氧化氢酶（CAT）活性则在100～400 mmol/L NaCl处理7 d和400 mmol/L NaCl处理1～7 d表现为先上升后降低的变化，在200 mmol/L胁迫7 d时最大；脯氨酸（Pro）、可溶性蛋白（SP）和可溶性糖（SS）含量随NaCl处理浓度增大和胁迫时间的延长逐渐增加，但仅高浓度、长时间NaCl处理（400 mmol/L处理7 d）下的SS含量显著高于对照；在200～400 mmol/L NaCl处理下，随胁迫时间的增加，‘大同黄花’赤霉素（GA）和脱落酸（ABA）含量先增后减，生长素（IAA）则表现为持续增加。在长时间（7 d） NaCl处理下，GA、ABA和IAA含量也表现出相同的变化趋势，在200 mmol/L NaCl处理下GA、ABA含量达到最大值，IAA含量则在400 mmol/L NaCl处理下最大。在‘大同黄花’综合评价中，200 mmol/L NaCl浓度‘大同黄花’隶属均值最大，100 mmol/L浓度次之，表明较低浓度NaCl胁迫下，‘大同黄花’能够通过控制生物量、调节抗氧化酶活性、内源激素和渗透物质含量，以提高机体耐盐性。本研究为不同程度盐渍地区的‘大同黄花’种植布局提供一定的理论依据。更多还原

【Abstract】 The effect of salt（NaCl） stress on the growth and physiology of Hemerocallis citrina ’Datong Huanghua’was explored. Hydroponics was used to study the effects of different concentrations of NaCl stress（0, 100, 200,400 mmol/L） and stress time（0, 1, 3, 5, 7 d） on the growth morphology, biomass, antioxidant enzyme activities,endogenous hormones and osmotic adjustment substances. The results showed that the fresh weight, dry weight of stems and leaves, leaf length and leaf width of seedlings decreased gradually with the increase of NaCl concentration. The fresh and dry weight of root increased first and then decreased. With the increase of NaCl concentration and stress time, the contents of superoxide anion free radical（O₂^-·）, hydrogen peroxide（H₂O₂） and malondialdehyde（MDA） in ’Datong Huanghua’ increased significantly. The contents of O₂^-·, H₂O₂ and MDA in 400 mmol/L NaCl treatment for 7 days were significantly higher than those in the control. With the increase of NaCl concentration,the activities of peroxidase（POD） and superoxide dismutase（SOD） increased first and then decreased, which was the largest under 200 mmol/L NaCl treatment. With the increase of stress time, the activities of POD and SOD continued to increase. The activity of catalase（CAT） increased first and then decreased under 100～400 mmol/L NaCl treatment for 7 days and 400 mmol/L NaCl treatment for 1～7 d, reaching the maximum under 200 mmol/L NaCl treatment for 7 days. The contents of proline（Pro）, soluble protein（SP） and soluble sugar（SS） increased gradually with the increase of NaCl concentration and stress time, but the content of SS in high concentration and long-term NaCl treatment（400 mmol/L treatment for 7 days） was significantly higher than that in the control. Under200～400 mmol/L NaCl treatment, with the increase of stress time, the contents of gibberellin（GA） and abscisic acid（ABA） in the leaves of ’Datong Huanghua’ increased first and then decreased, while indole-3-acetic acid（IAA）increased continuously. The contents of GA, ABA and IAA also showed the same change trend under long-term（7 days） NaCl treatment. The contents of GA and ABA reached the maximum under 200 mmol/L NaCl treatment,while the content of IAA reached the maximum under 400 mmol/L NaCl treatment. In the comprehensive evaluation of ’Datong Huanghua’, the mean value of ’Datong Huanghua’ under 200 mmol/L NaCl treatment was the largest, followed by 100 mmol/L concentration, indicating that ’Datong Huanghua’ could improve the salt tolerance by controlling biomass, regulating antioxidant enzyme activity, endogenous hormones and osmotic substance content under low concentration NaCl stress. This study provided a theoretical basis for the planting layout of ’Datong Huanghua’ in saline areas with different degrees.更多还原