【摘要】 普通油茶是中国重要的木本油料树种，ARF基因家族在植物生长发育和逆境胁迫中起关键作用。为了更加深入了解普通油茶ARF基因家族的特征，本研究以普通油茶基因组信息为研究对象，利用生物信息学方法鉴定普通油茶的CoARF基因家族成员，并对其结构、理化性质、保守结构域、系统进化树和顺式作用元件进行分析。结果表明：(1)共鉴定出25个ARF基因，分别命名为CoARF1～CoARF25，其蛋白长度范围在372～1 085个氨基酸之间；分子量在41 147.74～119 606.64 Da之间；等电点为5.44～8.53;(2) 25个普通油茶ARF基因在染色体3、4、6、7、9、10、11、13和15上成簇分布或单个分布，且分布不均匀；(3) CoARFs基因家族成员均含有内含子，数量为1～15个之间；其CoARFs均含有B3结构域和中间的Auxin＿resp结构域，而只有部分成员含有AUX＿IAA碳末端结构域；(4)进化树结果表明，ARF基因家族分为4个亚族(ClassⅠa,ClassⅠb, ClassⅡ, ClassⅢ)中，不同亚家族中CoARF成员数目有所差异；(5) CoARFs基因上游调控区域检测到35种顺式作用元件共551个，其顺式作用元件主要包括茉莉酸、脱落酸、赤霉素、水杨酸和生长素等激素的响应元件及低温、干旱、防御与胁迫、厌氧诱导和光照响应等逆境相应元件。本研究为探索油茶ARF基因家族成员的功能提供一定的参考。更多还原

【Abstract】 Camellia oleifera is a very important oily-tree species with higher economic and ecological value. ARF genes play vital roles in development and environmental stress of plants. In order to understand ARF gene family in C. oleifera well, CoARF genes were identified and the analysis of their structures, phylogenetics, conserved domains, physical and chemical characteristics and cis-acting elements were carried out. The results showed that:(1)Twenty-five CoARF genes were identified and named by CoARF1～CoARF25, respectively; their molecular weights ranged from 41 147.74 to 119 606.64 Da; the isoelectric points ranged from 5.44 to 8.53.(2) The 25 ARF genes of C.oleifera are distributed in clusters or singly on chromosomes 3, 4, 6, 7, 9, 10, 11, 13, and 15, and the distribution is uneven.(3) All CoARFs had introns whose number ranged from 1 to 15; every member of CoARFs had the B3 domain and AUX＿resp domain in the middle region, however only some members had carboxy-terminal domains of AUX＿IAA.(4) The CoARF family fell into 4 classes by the combined phylogenetic analysis, the number of members was various in different classes.(5) Five hundred and fifty-one cis-acting elements of 35 types detected were divided two broad classes, one class included the cis-acting elements of jasmonic acid, abscisic acid, gibberellin,salicylic acid and auxin; another class was the cis-acting element collection of low-temperature, drought, defense and stress, anaerobic induction and light. This will provide a solid foundation for the further function studies of CoARFs gene family and genetic improvement of C. oleifera.更多还原