【摘要】 WRKY转录因子在植物代谢及抗逆过程中起着重要的作用。本研究为探明PtWRKY21基因在毛果杨中的功能，克隆了PtrWRKY21基因，并对其进行了生物信息学分析。结果如下：提取了毛果杨叶片中的总RNA，并反转录成cDNA，经过PCR克隆技术得到了与毛果杨基因组数据库中公布的一致的基因序列。对该基因进行了生物信息学分析，确定了PtrWRKY21基因所编码的蛋白质氨基酸等电点为9.64，氨基酸数量为347个，分子量为38 556.76 Da，是一种亲水性稳定蛋白质。其二级结构由24.78%α-螺旋，61.38%无规卷曲，9.80%延伸链，4.03%β-折叠组成。三级结构基本与二级结构一致。另外，该基因与小垫柳亲缘关系较近。本研究开展了对PtrWRKY21的克隆及生物信息学分析研究，为阐明PtrWRKY21基因的功能提供参考。更多还原

【Abstract】 WRKY transcription factors play an important role in plant metabolism and stress resistance. In order to explore the function of PtWRKY21 gene in Populus trichocarpa, PtrWRKY21 gene was cloned and analyzed by bioinformatics. The results were list as follows: total RNA was extracted from the leaves of P. trichocarpa and reversely transcribed into cDNA. The gene sequence was obtained by PCR cloning technology which was consistent with that published in P. trichocarpa genome database. Bioinformatic analysis was performed on the gene, and it was determined that the protein encoded by the PtrWRKY21 gene has an isoelectric point of 9.64, consists of 347 amino acids, has a molecular weight of 38 556.76 Da, and is a hydrophilic stable protein. Its secondary structure consists of 24.78% α-helix, 61.38% random coil, 9.80% extended strand and 4.03% β-sheet. Tertiary structure is basically inhibited with secondary structure. In addition, they are closely related to their relatives. The cloning and bioinformatics analysis of PtrWRKY21 in this study can provide a reference for further research on the function of the PtrWRKY21 gene.更多还原